ArticleCommunications biology2018
In situ serial crystallography for rapid de novo membrane protein structure determination.
Article in Communications biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- An integrated experimental and computational pipeline for crystallographic fragment screening of membrane protein in the lipid cubic phase.Communications chemistry · 2026Article
- Advances in Crystallography and Cryo-Electron Microscopy for the Study of Membrane Proteins.Advances in experimental medicine and biology · 2026Review
- Evolution of macromolecular crystallography beamlines at the Swiss Light Source and SwissFEL.Journal of synchrotron radiation · 2025Article
- In situ serial crystallography facilitates 96-well plate structural analysis at low symmetry.IUCrJ · 2024Article
- Article
- Integrative analysis of pathogenic variants in glucose-6-phosphatase based on an AlphaFold2 model.PNAS nexus · 2024Article
- Microfluidic rotating-target device capable of three-degrees-of-freedom motion for efficient in situ serial synchrotron crystallography.Journal of synchrotron radiation · 2023Article
- Article
- An automated platform for structural analysis of membrane proteins through serial crystallography.Cell reports methods · 2021Article
- Versatile microporous polymer-based supports for serial macromolecular crystallography.Acta crystallographica. Section D, Structural biology · 2021Article
- Structural basis of the membrane intramolecular transacylase reaction responsible for lyso-form lipoprotein synthesis.Nature communications · 2021Article
- The crystal structure of bromide-boundeLife · 2021Article
- Changes in Membrane Protein Structural Biology.Biology · 2020Review
- Structure, dynamics and lipid interactions of serotonin receptors: excitements and challenges.Biophysical reviews · 2020Review
- Low-doseIUCrJ · 2020Article
- Structure Determination from Lipidic Cubic Phase Embedded Microcrystals by MicroED.Structure (London, England : 1993) · 2020Article
- Making a difference in multi-data-set crystallography: simple and deterministic data-scaling/selection methods.Acta crystallographica. Section D, Structural biology · 2020Article
- 3D-printed holders forJournal of applied crystallography · 2020Article
- Article
- Article
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Authors and funding
11 authors.
Funding
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Abstract
De novo membrane protein structure determination is often limited by the availability of large crystals and the difficulties in obtaining accurate diffraction data for experimental phasing. Here we present a method that combines in situ serial crystallography with de novo phasing for fast, efficient membrane protein structure determination. The method enables systematic diffraction screening and rapid data collection from hundreds of microcrystals in in meso crystallization wells without the need for direct crystal harvesting. The requisite data quality for experimental phasing is achieved by accumulating diffraction signals from isomorphous crystals identified post-data collection. The method works in all experimental phasing scenarios and is particularly attractive with fragile, weakly diffracting microcrystals. The automated serial data collection approach can be readily adopted at most microfocus macromolecular crystallography beamlines.
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