ArticleCommunications biology2018
Rapid and gentle hydrogel encapsulation of living organisms enables long-term microscopy over multiple hours.
Article in Communications biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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The trial behind it
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Who cites it
39 citing papers in PubMed.
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- Neuronal Dynamics During Isoflurane Induction inbioRxiv : the preprint server for biology · 2026Article
- The volatile anesthetic isoflurane causes global suppression of neuronal activity, disrupting hub neuron function inFrontiers in systems neuroscience · 2026Article
- Present and future of smart functional materials as actuators in microfluidic devices.Lab on a chip · 2025Review
- Three types of actomyosin rings within a common cytoplasm exhibit distinct modes of contractility.Molecular biology of the cell · 2025Article
- Three types of actomyosin rings within a common cytoplasm exhibit distinct modes of contractility.bioRxiv : the preprint server for biology · 2025Article
- A novel indicator-based visualisation method to investigate diffusion behaviour of dissolved COMethodsX · 2025Article
- Visualization and Analyses of Cytoplasmic Streaming in C. elegans Zygotes.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Development of a size-separation technique to isolate Caenorhabditis elegans embryos using mesh filters.PloS one · 2025Article
- Applications of Lightsheet Fluorescence Microscopy by High Numerical Aperture Detection Lens.The journal of physical chemistry. B · 2024Review
- 3D Printed Organisms Enabled by Aspiration-Assisted Adaptive Strategies.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Applications of genetic code expansion technology in eukaryotes.Protein & cell · 2024Review
- Worm-Based Diagnosis Combining Microfluidics toward Early Cancer Screening.Micromachines · 2024Review
- Polyethylene glycol as an improved barrier to prevent fleeing inmicroPublication biology · 2024Article
- The homeodomain transcriptional regulator DVE-1 directs a program for synapse elimination during circuit remodeling.Nature communications · 2023Article
- Modulation of Methacrylated Hyaluronic Acid Hydrogels Enables Their Use as 3D Cultured Model.Gels (Basel, Switzerland) · 2023Article
- Measures of Information Content during Anesthesia and Emergence in the Caenorhabditis elegans Nervous System.Anesthesiology · 2023Article
- Visualizing Neurons Under Tension In Vivo with Optogenetic Molecular Force Sensors.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Live Imaging in Planarians: Immobilization and Real-Time Visualization of Reactive Oxygen Species.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Live-Cell Imaging of Intact Ex Vivo Globes Using a Novel 3D Printed Holder.Journal of visualized experiments : JoVE · 2022Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Imaging living organisms at high spatial resolution requires effective and innocuous immobilization. Long-term imaging places further demands on sample mounting with minimal perturbation of the organism. Here we present a simple, inexpensive method for rapid encapsulation of small animals of any developmental stage within a photo-crosslinked polyethylene glycol (PEG) hydrogel, gently restricting movement within their confined spaces. Immobilized animals maintain their original morphology in a hydrated environment compatible with chemical treatment, optical stimulation, and light-sheet microscopy. We demonstrate prolonged three-dimensional imaging of neural responses in the nematode
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.