Evidence map›Paper›PMID 30271869›Full record

ArticleRegenerative therapy2018

Respective optimal calcium concentrations for proliferation on type I collagen fibrils in two keratinocyte line cells, HaCaT and FEPE1L-8.

Hitomi Fujisaki, Sugiko Futaki, Masashi Yamada, Kiyotoshi Sekiguchi, Toshihiko Hayashi, Takashi Ikejima, Shunji Hattori

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Regenerative therapy, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
0.8field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 25 citations in OpenAlex.

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  11. Water-Soluble Fullerenol CInternational journal of molecular sciences · 2019
    Article
  12. Differential Effects of Calcium Alginate and Carboxymethylcellulose Wound Dressing Extracts on Human Sensory Neuron Regeneration and Secretome.Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 2 countries.

Hitomi FujisakiNippi Research Institute of Biomatrix, Toride, Ibaraki, Japan.
Sugiko FutakiDepartment of Anatomy and Cell Biology, Osaka Medical College, Osaka, Japan.
Masashi YamadaDivision of Matrixome Research and Application, Institute for Protein Research, Osaka University, Suita, Osaka, Japan.
Kiyotoshi SekiguchiDivision of Matrixome Research and Application, Institute for Protein Research, Osaka University, Suita, Osaka, Japan.
Toshihiko HayashiChina-Japan Research Institute of Medical and Pharmaceutical Sciences, Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang, China.
Takashi IkejimaChina-Japan Research Institute of Medical and Pharmaceutical Sciences, Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang, China.
Shunji HattoriNippi Research Institute of Biomatrix, Toride, Ibaraki, Japan.
Japan Institute of Leather Research · JPOsaka University · JPShenyang Pharmaceutical University · CNOsaka Medical and Pharmaceutical University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Keratinocyte line cells HaCaT and FEPE1L-8 are used for skin model with type I collagen fibrils (gels). For this purpose, not only differentiation but also regulation of proliferation on type I collagen gels by exogenous calcium concentration is important. When exogenous calcium concentration is low, primary keratinocyte proliferation is repressed and eventually cells are induced to apoptosis on type I collagen gels. The apoptosis induced on type I collagen gels is suppressed by increasing calcium concentration in the medium. That is, higher exogenous calcium concentration is necessary for primary keratinocyte survival on type I collagen gels than for that on dish surface culture. Meanwhile much higher exogenous calcium causes cell differentiation and inhibition of proliferation. The optimal calcium concentrations for proliferation on type I collagen gels have not been clarified in keratinocyte line cells. HaCaT cells have a unique calcium sensitivity in comparison with primary keratinocytes, whereas FEPE1L-8 cells have a similar sensitivity to primary keratinocytes. In this study, we compared the effect of calcium concentrations on proliferation of HaCaT and FEPE1L-8 cells on type I collagen gels. On type I collagen gels, both line cells required higher calcium concentrations for proliferation than on dish surface. HaCaT cells proliferated better in a wider range of calcium concentrations than FEPE1L-8 cells.

Indexed as

Calcium concentrationDAG, diacylglycerolDMEM (0), DMEM supplemented without fetal bovine serumDMEM (10), DMEM supplemented with 10% fetal bovine serumDMEM, Dulbecco's Modified Eagle's MediumECM, extracellular matrixHBSS, Hanks' balanced salt solutionHEPES, 4-(2-hydroxyethyl-1-piperazineethanesulfonic acidIP3, inositol trisphosphateK110, K110 type II mediumKeratinocyte proliferationMTT, 3-(4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazoliumbromidePI3K, phosphoinositide 3-OH-kinasePIP2, hydrolyze phosphatidylinositol bisphosphatePI, propidium iodidePKC, protein kinase CType I collagen gelWST-8, (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt

Identifiers

PMID30271869
PMCPMC6146901
OpenAlexW2804009326

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.