ArticleGreen chemistry : an international journal and green chemistry resource : GC2017
Long-term protein packaging in bio-ionic liquids: Improved catalytic activity and enhanced stability of cytochrome C against multiple stresses.
Article in Green chemistry : an international journal and green chemistry resource : GC, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 107 citations in OpenAlex.
- Stoichiometrically Engineered Hydrated Ionic Liquids Enabling Reinforcement of Enzyme Cascade with Improved Thermodynamic Stability.ACS sustainable chemistry & engineering · 2026Article
- Enzyme-Support Interactions ofACS applied materials & interfaces · 2025Article
- Cholinium-Based Ionic Liquids Modulate Protein Stability: A Comparative Study of Enzymes and Albumins.Molecules (Basel, Switzerland) · 2025Article
- Understanding the Effect of Ionic Liquid-Mediated Solvent Engineering on the Kinetics and Thermodynamic Stability of Phenylalanine Ammonia-Lyase.The journal of physical chemistry. B · 2024Article
- Do Ionic Liquids Exhibit the Required Characteristics to Dissolve, Extract, Stabilize, and Purify Proteins? Past-Present-Future Assessment.Chemical reviews · 2024Review
- Extending the computational and experimental analysis of lipase active site selectivity.Bioprocess and biosystems engineering · 2024Article
- A Comprehensive Review on Imperative Role of Ionic Liquids in Pharmaceutical Sciences.Current drug delivery · 2024Review
- Investigating BioCaRGOS, a Sol-Gel Matrix for the Stability of Heme Proteins under Enzymatic Degradation and Low pH.ACS omega · 2023Article
- Cytochrome c in cancer therapy and prognosis.Bioscience reports · 2022Article
- Ionic Liquids: Promising Approach for Oral Drug Delivery.Pharmaceutical research · 2022Review
- Lysine-PEGylated Cytochrome C with Enhanced Shelf-Life Stability.Biosensors · 2022Article
- Computational and experimental analysis on the preferential selectivity of lipases for triglycerides in Licuri oil.Bioprocess and biosystems engineering · 2021Article
- Applications of Ionic Liquids in Whole-Cell and Isolated Enzyme Biocatalysis.Molecules (Basel, Switzerland) · 2021Review
- Characterization of Ionic Liquid Lignins Isolated from Spruce Wood with 1-Butyl-3-methylimidazolium Acetate and Methyl Sulfate and Their Binary Mixtures with DMSO.Molecules (Basel, Switzerland) · 2020Article
- Ionic liquids as biocompatible stabilizers of proteins.Biophysical reviews · 2018Review
- Innovative aspects of protein stability in ionic liquid mixtures.Biophysical reviews · 2018Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
There is a considerable interest in the use of structurally stable and catalytically active enzymes, such as cytochrome C (Cyt C), in the pharmaceutical and fine chemical industries. However, harsh process conditions, such as temperature, pH, and presence of organic solvents, are the major barriers to the effective use of enzymes in biocatalysis. Herein, we demonstrate the suitability of bio-based ionic liquids (ILs) formed by the cholinium cation and dicarboxylate-based anions as potential media for enzymes, in which remarkable enhanced activity and improved stability of Cyt C against multiple stresses were obtained. Among the several bio-ILs studied, an exceptionally high catalytic activity (> 50-fold) of Cyt C was observed in aqueous solutions of cholinium glutarate ([Ch][Glu]; 1g/mL) as compared to the commonly used phosphate buffer solutions (pH 7.2), and > 25-fold as compared to aqueous solutions of cholinium dihydrogen phosphate ([Ch][Dhp]; 0.5g/mL) -the best known IL for long term stability of Cyt C. The catalytic activity of the enzyme in presence of bio-ILs was retained against several external stimulus, such as chemical denaturants (H
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.