ArticleJournal of muscle research and cell motility1986
Partial purification of two myosin heavy chain kinases from Dictyostelium discoideum.
Article in Journal of muscle research and cell motility, 1986. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
8 citing papers in PubMed, 22 citations in OpenAlex.
- Myosin light chain kinase (MLCK) gene disruption in Dictyostelium: a role for MLCK-A in cytokinesis and evidence for multiple MLCKs.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Release of myosin II from the membrane-cytoskeleton of Dictyostelium discoideum mediated by heavy-chain phosphorylation at the foci within the cortical actin network.The Journal of cell biology · 1992Article
- Stopped-flow measurement of cytoskeletal contraction: Dictyostelium myosin II is specifically required for contraction of amoeba cytoskeletons.The Journal of cell biology · 1991Article
- Dictyostelium discoideum myosin: isolation and characterization of cDNAs encoding the regulatory light chain.Molecular and cellular biology · 1989Article
- Molluscan muscle. Where's the catch?Journal of muscle research and cell motility · 1988Article
- Dictyostelium discoideum myosin: isolation and characterization of cDNAs encoding the essential light chain.Molecular and cellular biology · 1988Article
- Effect of heavy chain phosphorylation on the polymerization and structure of Dictyostelium myosin filaments.The Journal of cell biology · 1987Article
- Myosin specific phosphatases isolated from Dictyostelium discoideum.Journal of muscle research and cell motility · 1986Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
Myosin heavy chain kinase activity was identified in the high speed supernate of lysed Dictyostelium amoebae and was precipitated by 30-50% ammonium sulphate. In low ionic strength buffer, the activity bound tightly to a Cibacron Blue Sepharose column and eluted as a single peak with 1.0 M NaCl. Gel filtration chromatography resolved the kinase into two activities, each of which phosphorylated the tail portion of purified Dictyostelium myosin. One of these activities phosphorylated both serine and threonine residues of the heavy chain, while the other activity only phosphorylated threonine residues. Peptide mapping studies indicated that in vivo and in vitro phosphorylation sites were identical. The heavy chain kinases required Mg2+ for activity but were unaffected by Ca2+ or calmodulin. The heavy chain kinases did not phosphorylate Dictyostelium light chain, and also did not phosphorylate myosins from striated, smooth, or other nonmuscle sources.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.