ArticleMicrobiologyOpen2019
Culturing marine bacteria from the genus Pseudoalteromonas on a cotton scaffold alters secondary metabolite production.
Article in MicrobiologyOpen, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Membrane Vesicle-Mediated Delivery of Antibacterial Lipopeptides byACS chemical biology · 2026Article
- Advances in natural product discovery: strategies, technologies, and insights.Natural products and bioprospecting · 2026Review
- Biosynthetic mechanism of the yellow pigments in the marine bacteriumApplied and environmental microbiology · 2024Article
- Natural Pigments Production and Their Application in Food, Health and Other Industries.Nutrients · 2023Review
- An extensive review of marine pigments: sources, biotechnological applications, and sustainability.Aquatic sciences · 2023Review
- Molecular Relationships in Biofilm Formation and the Biosynthesis of Exoproducts in Pseudoalteromonas spp.Marine biotechnology (New York, N.Y.) · 2022Review
- Fungal Endophytes: A Potential Source of Antibacterial Compounds.Journal of fungi (Basel, Switzerland) · 2022Review
- Cybergenetic control of microbial community composition.Frontiers in bioengineering and biotechnology · 2022Review
- Inhibition of Isoleucyl-tRNA Synthetase by the Hybrid Antibiotic Thiomarinol.Journal of the American Chemical Society · 2021Article
- Solid-Phase Extraction Embedded Dialysis (SPEED), an Innovative Procedure for the Investigation of Microbial Specialized Metabolites.Marine drugs · 2021Article
- Addition of insoluble fiber to isolation media allows for increased metabolite diversity of lab-cultivable microbes derived from zebrafish gut samples.Gut microbes · 2020Article
- Isolation and characterization of violacein from an Antarctic Iodobacter: a non-pathogenic psychrotolerant microorganism.Extremophiles : life under extreme conditions · 2020Article
- Advances in microbial culturing conditions to activate silent biosynthetic gene clusters for novel metabolite production.Journal of industrial microbiology & biotechnology · 2019Review
- Culturing marine bacteria from the genus Pseudoalteromonas on a cotton scaffold alters secondary metabolite production.MicrobiologyOpen · 2019Article
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Authors and funding
4 authors.
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Abstract
The discovery of secondary metabolites from marine microorganisms is beset by numerous challenges including difficulties cultivating and subsequently eliciting expression of biosynthetic genes from marine microbes in the laboratory. In this paper, we describe a method of culturing three species from the marine bacterial genus Pseudoalteromonas using cotton scaffold supplemented liquid media. This simple cultivation method was designed to mimic the natural behavior of some members of the genus wherein they form epibiotic/symbiotic associations with higher organisms such as sponges and corals or attach to solid structures as a biofilm. Our scaffolded cultivation is highly effective at stimulating an attachment/biofilm phenotype and causes large changes to metabolite profiles for the microbes investigated. Metabolite changes include alteration to the production levels of known molecules such as violacein, thiomarinol A, and the alterochromide and prodiginine families of molecules. Finally and critically, our technique stimulates the production of unknown compounds that will serve as leads for future natural product discovery. These results suggest our cultivation approach could potentially be used as a general strategy for the activation of silent gene clusters in marine microbes to facilitate access to their full natural product biosynthetic capacity.
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