ArticleThe Journal of reproduction and development2018
Freezing sperm in short straws reduces storage space and allows transport in dry ice.
Article in The Journal of reproduction and development, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed, 10 citations in OpenAlex.
- Evaluation of Dry Ice for Short-Term Storage and Transportation of Frozen Boar Semen.Animals : an open access journal from MDPI · 2024Article
- Article
- Ketone body and FGF21 coordinately regulate fasting-induced oxidative stress response in the heart.Scientific reports · 2022Article
- Urinary FABP1 is a biomarker for impaired proximal tubular protein reabsorption and is synergistically enhanced by concurrent liver injury.The Journal of pathology · 2021Article
- Improvement of short straws for sperm cryopreservation: installing an air-permeable filter facilitates handling.The Journal of reproduction and development · 2021Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Efficient cryopreservation and transportation of mouse sperm are among the most desirable strategies for current and future research on mouse genetics. However, the current method for sperm cryopreservation uses an 11-cm plastic straw, which is a bulky and fragile container. Developing an alternative to overcome the limitations associated with this method would accelerate biomedical research. Here, we developed the ST (sperm-freezing in ShorT STraw to reduce STorage space) method for cryopreserving mouse sperm in short 3.8-cm plastic straws. Up to nine short straws can be stored in a cryotube, reducing storage space. We further show that sperm frozen by the ST method can be transported in liquid nitrogen or dry ice without any detrimental effects on subsequent fertilization and the birth rate. Our findings suggest that this sperm-freezing method is beneficial not only for individual laboratories but also for large-scale mutagenesis/knockout and phenotyping programs.
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