ArticleBiosensors & bioelectronics2019
Quantification of cDNA on GMR biosensor array towards point-of-care gene expression analysis.
Article in Biosensors & bioelectronics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 48 citations in OpenAlex.
- Integrated Microfluidic Giant Magnetoresistance (GMR) Biosensor Platform for Magnetoresistive Immunoassay of Myoglobin.Biosensors · 2025Article
- Design and Validation of Specific Oligonucleotide Probes on Planar Magnetic Biosensors.Analytical chemistry · 2024Article
- Rapid, Point-of-Care Host-Based Gene Expression Diagnostics Using Giant Magnetoresistive Biosensors.ACS sensors · 2023Article
- Advances in point-of-care genetic testing for personalized medicine applications.Biomicrofluidics · 2023Review
- Magnetic Particle Spectroscopy for Point-of-Care: A Review on Recent Advances.Sensors (Basel, Switzerland) · 2023Review
- A GMR-based assay for quantification of the human response to influenza.Biosensors & bioelectronics · 2022Article
- Magnetic nanoparticles and magnetic particle spectroscopy-based bioassays: a 15 year recap.Nano futures · 2022Article
- From saliva to SNP: non-invasive, point-of-care genotyping for precision medicine applications using recombinase polymerase amplification and giant magnetoresistive nanosensors.Lab on a chip · 2022Article
- Multiplex Digital Quantification of β-Lactamase Genes in Antibiotic-Resistant Bacteria by Counting Gold Nanoparticle Labels on Silicon Microchips.Biosensors · 2022Article
- Giant Magnetoresistance Biosensors in Biomedical Applications.ACS applied materials & interfaces · 2022Review
- A plasmonic gold nanofilm-based microfluidic chip for rapid and inexpensive droplet-based photonic PCR.Scientific reports · 2021Article
- Performance Validation of a Planar Hall Resistance Biosensor through Beta-Amyloid Biomarker.Sensors (Basel, Switzerland) · 2020Article
- Advances in Magnetoresistive Biosensors.Micromachines · 2019Review
- Integrated microfluidic systems with sample preparation and nucleic acid amplification.Lab on a chip · 2019Review
- Quasi-Static Current Measurement with Field-Modulated Spin-Valve GMR Sensors.Sensors (Basel, Switzerland) · 2019Article
- Multichannel digital heteronuclear magnetic resonance biosensor.Biosensors & bioelectronics · 2019Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Gene expression analysis at the point-of-care is important for rapid disease diagnosis, but traditional techniques are limited by multiplexing capabilities, bulky equipment, and cost. We present a gene expression analysis platform using a giant magnetoresistive (GMR) biosensor array, which allows multiplexed transcript detection and quantification through cost-effective magnetic detection. In this work, we have characterized the sensitivity, dynamic range, and quantification accuracy of Polymerase chain reaction (PCR)-amplified complementary DNA (cDNA) on the GMR for the reference gene GAPDH. A synthetic GAPDH single-stranded DNA (ssDNA) standard was used to calibrate the detection, and ssDNA dilutions were qPCR-amplified to obtain a standard curve. We demonstrate that the GMR platform provides a dynamic range of 4 orders of magnitude and a limit of detection of 1 pM and 0.1 pM respectively for 15 and 18-cycle amplified synthetic GAPDH PCR products. The quantitative results of GMR analysis of cell-line RNA were confirmed by qPCR.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.