ArticleBiophysical journal2018
Microtubule Dynamics, Kinesin-1 Sliding, and Dynein Action Drive Growth of Cell Processes.
Article in Biophysical journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 28 citations in OpenAlex.
- Steady-state epithelial apical flatness is characterized by MLCK morphodynamics and asynchronous CaMolecular biology of the cell · 2026Article
- Non-transport functions of motor proteins in corticogenesis.Frontiers in cell and developmental biology · 2026Review
- Roles of molecular motors in insulin-secreting beta cells.Current opinion in cell biology · 2025Review
- Modeling actin-microtubule crosstalk in migrating cells.Biophysical journal · 2025Article
- Enhanced Single-Particle Upconversion Imaging via Energy Migration Boosting.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- The Extremely Low Mechanical Force Generated by Nano-Pulling Induces Global Changes in the Microtubule Network, Nuclear Morphology, and Chromatin Transcription in Neurons.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Microtubule polymerization generates microtentacles important in circulating tumor cell invasion.Biophysical journal · 2025Article
- Patronin regulates presynaptic microtubule organization and neuromuscular junction development iniScience · 2024Article
- A human proteogenomic-cellular framework identifies KIF5A as a modulator of astrocyte process integrity with relevance to ALS.Communications biology · 2023Article
- Unrestrained growth of correctly oriented microtubules instructs axonal microtubule orientation.eLife · 2022Article
- Protein friction and filament bending facilitate contraction of disordered actomyosin networks.Biophysical journal · 2021Article
- Mini-review: Microtubule sliding in neurons.Neuroscience letters · 2021Review
- Manipulation of Axonal Outgrowth via Exogenous Low Forces.International journal of molecular sciences · 2020Review
- Extremely Low Forces Induce Extreme Axon Growth.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Mechanical Regulation of Neurite Polarization and Growth: A Computational Study.Biophysical journal · 2020Article
- Article
- Unconventional Roles of Cytoskeletal Mitotic Machinery in Neurodevelopment.Trends in cell biology · 2019Review
- The quantification and regulation of microtubule dynamics in the mitotic spindle.Current opinion in cell biology · 2019Review
- Bidirectional sliding of two parallel microtubules generated by multiple identical motors.Journal of mathematical biology · 2019Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
Recent experimental studies of the role of microtubule sliding in neurite outgrowth suggested a qualitative model, according to which kinesin-1 motors push the minus-end-out microtubules against the cell membrane and generate the early cell processes. At the later stage, dynein takes over the sliding, expels the minus-end-out microtubules from the neurites, and pulls in the plus-end-out microtubules that continue to elongate the nascent axon. This model leaves unanswered a number of questions: why is dynein unable to generate the processes alone, whereas kinesin-1 can? What is the role of microtubule dynamics in process initiation and growth? Can the model correctly predict the rates of process growth in control and dynein-inhibited cases? What triggers the transition from kinesin-driven to dynein-driven sliding? To answer these questions, we combine computational modeling of a network of elastic dynamic microtubules and kinesin-1 and dynein motors with measurements of the process growth kinetics and pharmacological perturbations in Drosophila S2 cells. The results verify quantitatively the qualitative model of the microtubule polarity sorting and suggest that dynein-powered elongation is effective only when the processes are longer than a threshold length, which explains why kinesin-1 alone, but not dynein, is sufficient for the process growth. Furthermore, we show that the mechanism of process elongation depends critically on microtubule dynamic instability. Both modeling and experimental measurements show, surprisingly, that dynein inhibition accelerates the process extension. We discuss implications of the model for the general problems of cell polarization, cytoskeletal polarity emergence, and cell process protrusion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.