Evidence map›Paper›PMID 30268522›Full record

ArticleNeuropharmacology2018

Prolonged-access to cocaine induces distinct Homer2 DNA methylation, hydroxymethylation, and transcriptional profiles in the dorsomedial prefrontal cortex of Male Sprague-Dawley rats.

Kyle L Ploense, Xiang Li, Danay Baker-Andresen, Amanda E Carr, Nick Woodward, Jared Bagley, Karen K Szumlinski, Timothy W Bredy, Tod E Kippin

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neuropharmacology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.6field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 13 citations in OpenAlex.

  1. Review
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  5. Article
  6. Epigenetics of addiction.Neurochemistry international · 2021
    Review
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Kyle L PloenseDepartment of Psychological & Brain Sciences, University of California, Santa Barbara, CA, USA; Institute for Collaborative Biotechnology, University of California, Santa Barbara, CA, USA; Department of Chemistry & Biochemistry, University of California, Santa Barbara, CA, USA. Electronic address: kploense@ucsb.edu.
Xiang LiQueensland Brain Institute, University of Queensland, St Lucia, QLD, Australia.
Danay Baker-AndresenQueensland Brain Institute, University of Queensland, St Lucia, QLD, Australia.
Amanda E CarrDepartment of Psychological & Brain Sciences, University of California, Santa Barbara, CA, USA.
Nick WoodwardDepartment of Psychological & Brain Sciences, University of California, Santa Barbara, CA, USA.
Jared BagleyDepartment of Psychological & Brain Sciences, University of California, Santa Barbara, CA, USA; Department of Psychology, Binghamton University, Binghamton, NY, USA.
Karen K SzumlinskiDepartment of Psychological & Brain Sciences, University of California, Santa Barbara, CA, USA; Neuroscience Research Institute, University of California, Santa Barbara, CA, USA; Department of Molecular Cellular Developmental Biology, University of California, Santa Barbara, CA, USA.
Timothy W BredyQueensland Brain Institute, University of Queensland, St Lucia, QLD, Australia.
Tod E KippinDepartment of Psychological & Brain Sciences, University of California, Santa Barbara, CA, USA; Neuroscience Research Institute, University of California, Santa Barbara, CA, USA; Department of Molecular Cellular Developmental Biology, University of California, Santa Barbara, CA, USA; Institute for Collaborative Biotechnology, University of California, Santa Barbara, CA, USA.
University of California, Santa Barbara · USUniversity of Queensland · AUBinghamton University · US

Funding

Interactions between prenatal stress and genetics in cocaine responsiveness.R01DA027525 · NIDA · UNIVERSITY OF CALIFORNIA SANTA BARBARA · PI KIPPIN, TOD EDWARD · 2010 to 2014
$1.6M
Homer-mediated signaling and cocaine addictionR01DA024038 · NIDA · UNIVERSITY OF CALIFORNIA SANTA BARBARA · PI SZUMLINSKI, KAREN KATHLEEN · 2008 to 2012
$1.5M
Chromatin Remodeling in the Prefrontal Cortex in Cocaine AddictionR21DA027115 · NIDA · UNIVERSITY OF CALIFORNIA SANTA BARBARA · PI KIPPIN, TOD EDWARD · 2010 to 2011
$411k
NIDA NIH HHS R01 DA024038NIDA NIH HHS R01 DA027525NIDA NIH HHS R21 DA027115
6 · The paper itself

Abstract

Repeated cocaine administration induces many long-term structural and molecular changes in the dorsal medial prefrontal cortex (dmPFC) and are known to underlie aspects of cocaine-seeking behavior. DNA methylation is a key long-lasting epigenetic determinant of gene expression and is implicated in neuroplasticity, however, the extent to which this epigenetic modification is involved in the neuroplasticity associated with drug addiction has received limited attention. Here, we examine the relation between DNA methylation and gene expression within the dorsal medial prefrontal cortex (dmPFC) following limited cocaine self-administration (1 h/day), prolonged cocaine self-administration (6 h/day), and saline self-administration (1 h/day). Rats were fitted with intravenous catheters and allowed to lever press for saline or cocaine (0.25 mg/kg/0.1 mL infusion) in the different access conditions for 20 days. Prolonged-access rats exhibited escalation in cocaine intake over the course of training, while limited-access rats did not escalate cocaine intake. Additionally, limited-access and prolonged-access rats exhibited unique Homer2 epigenetic profiles and mRNA expression. In prolonged-access rats, Homer2 mRNA levels in the dmPFC were increased, which was accompanied by decreased DNA methylation and p300 binding within the Homer2 promoter. Limited-access animals exhibited decreased DNA methylation, decreased DNA hydroxymethylation, and increased p300 binding within the Homer2 promoter. These data indicate that distinct epigenetic profiles are induced by limited-versus prolonged-access self-administration conditions that contribute to transcriptional profiles and lend support to the notion that covalent modification of DNA is implicated in addiction-like changes in cocaine-seeking behavior.

Indexed as

AnimalsCocaineCocaine-Related DisordersDNA MethylationDopamine Uptake InhibitorsDrug-Seeking BehaviorE1A-Associated p300 ProteinEpigenesis, GeneticHomer Scaffolding ProteinsMalePrefrontal CortexPromoter Regions, GeneticRats, Sprague-DawleyRNA, MessengerSelf AdministrationTranscriptomeCocaineDopamine Uptake InhibitorsE1A-Associated p300 ProteinEp300 protein, ratHomer2 protein, ratHomer Scaffolding ProteinsRNA, MessengerCocaineDNA methylationEpigeneticsHomer2mRNAPrefrontal cortexSelf-administration

Identifiers

PMID30268522
PMCPMC6371788
OpenAlexW2893077742

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.