ArticleiScience2018
Two-Photon Optogenetic Mapping of Excitatory Synaptic Connectivity and Strength.
Article in iScience, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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Who cites it
11 citing papers in PubMed.
- High-throughput synaptic connectivity mapping using in vivo two-photon holographic optogenetics and compressive sensing.Nature neuroscience · 2025Article
- Multiphoton Neurophotonics: Recent Advances in Imaging and Manipulating Neuronal Circuits.ACS photonics · 2025Review
- Principles of visual cortex excitatory microcircuit organization.Innovation (Cambridge (Mass.)) · 2025Article
- Two-photon optogenetics-based assessment of neuronal connectivity in healthy and chronic hypoperfusion mice.Neurophotonics · 2024Article
- Removing direct photocurrent artifacts in optogenetic connectivity mapping data via constrained matrix factorization.PLoS computational biology · 2024Article
- Modern Methods for Unraveling Cell- and Circuit-Level Mechanisms of Neurophysiological Biomarkers in Psychiatry.Advances in neurobiology · 2024Review
- Determinants of functional synaptic connectivity among amygdala-projecting prefrontal cortical neurons in male mice.Nature communications · 2023Article
- Synaptic connectivity to L2/3 of primary visual cortex measured by two-photon optogenetic stimulation.eLife · 2022Article
- A Platform for Spatiotemporal "Matrix" Stimulation in Brain Networks Reveals Novel Forms of Circuit Plasticity.Frontiers in neural circuits · 2021Article
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Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The development of optical methods to activate neurons with single-cell resolution has enabled systematic mapping of inhibitory connections. In contrast, optical mapping of excitatory connections between pyramidal neurons (PCs) has been a major challenge due to their high densities in cortical tissue and their weak and stochastic connectivity. Here we present an optogenetic two-photon mapping method in mouse neocortical slices by activating PCs with the red-shifted opsin C1V1 while recording postsynaptic responses in whole-cell configuration. Comparison of delays from triggered action potentials (APs) with those from synaptic inputs allowed us to predict connected PCs in three dimensions. We confirmed these predictions with paired recordings, and used this method to map strong connections among large populations of layer 2/3 PCs. Our method can be used for fast, systematic mapping of synaptic connectivity and weights.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.