ArticlePlant biotechnology journal2019
Simultaneous regulation of F5H in COMT-RNAi transgenic switchgrass alters effects of COMT suppression on syringyl lignin biosynthesis.
Article in Plant biotechnology journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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48 citing papers in PubMed, 112 citations in OpenAlex.
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- Genome and transcriptomics provide insights on stipular spine morphogenesis inForestry research · 2026Article
- Metabolic engineering strategies for optimized lignan production in plants.Frontiers in plant science · 2026Review
- A functional cascading of lignin modification via repression of caffeic acid O-methyltransferase for bioproduction and anti-oxidation in rice.Journal of advanced research · 2025Article
- Lignin: the primary component responsible for endocarp sclerosis in the development of Cornus officinalis fruit.BMC plant biology · 2025Article
- The telomere-to-telomere genome of Pucai () (Horticulture research · 2025Article
- CRISPR/Cas9-mediated disruption of DA1 enhances both biomass yield and quality in poplar.Plant biotechnology journal · 2025Article
- Direct and indirect effects of multiplex genome editing of F5H and FAD2 in oil crop camelina.Plant biotechnology journal · 2025Article
- Study on the mechanism of exogenous CaClFrontiers in plant science · 2025Article
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- Lr34/Yr18/Sr57/Pm38 confers broad-spectrum resistance to fungal diseases via sinapyl alcohol transport for cell wall lignification in wheat.Plant communications · 2024Article
- Metabolomics and proteomics analyses of Chrysanthemi Flos: a mechanism study of changes in proteins and metabolites by processing methods.Chinese medicine · 2024Article
- Genome-Wide Identification of thePlants (Basel, Switzerland) · 2024Article
- Exploring Lignin Biosynthesis Genes in Rice: Evolution, Function, and Expression.International journal of molecular sciences · 2024Article
- UDP-glycosyltransferase UGT96C10 functions as a novel detoxification factor for conjugating the activated dinitrotoluene sulfonate in switchgrass.Plant biotechnology journal · 2024Article
- A Combined Metabolome and Transcriptome Reveals the Lignin Metabolic Pathway during the Developmental Stages of Peel Coloration in the 'Xinyu' Pear.International journal of molecular sciences · 2024Article
- Evolution and Analysis of Caffeic Acid Transferase (COMT) in Seed Plants.Biochemical genetics · 2024Article
- Blocking miR396 activity by overexpression MIM396 improved switchgrass tiller number and biomass yield.Biotechnology for biofuels and bioproducts · 2024Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ferulate 5-hydroxylase (F5H) catalyses the hydroxylation of coniferyl alcohol and coniferaldehyde for the biosynthesis of syringyl (S) lignin in angiosperms. However, the coordinated effects of F5H with caffeic acid O-methyltransferase (COMT) on the metabolic flux towards S units are largely unknown. We concomitantly regulated F5H expression in COMT-down-regulated transgenic switchgrass (Panicum virgatum L.) lines and studied the coordination of F5H and COMT in lignin biosynthesis. Down-regulation of F5H in COMT-RNAi transgenic switchgrass plants further impeded S lignin biosynthesis and, consequently, increased guaiacyl (G) units and reduced 5-OH G units. Conversely, overexpression of F5H in COMT-RNAi transgenic plants reduced G units and increased 5-OH units, whereas the deficiency of S lignin biosynthesis was partially compensated or fully restored, depending on the extent of COMT down-regulation in switchgrass. Moreover, simultaneous regulation of F5H and COMT expression had different effects on cell wall digestibility of switchgrass without biomass loss. Our results indicate that up-regulation and down-regulation of F5H expression, respectively, have antagonistic and synergistic effects on the reduction in S lignin resulting from COMT suppression. The coordinated effects between lignin genes should be taken into account in future studies aimed at cell wall bioengineering.
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