ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2018
Implications of stx loss for clinical diagnostics of Shiga toxin-producing Escherichia coli.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 25 citations in OpenAlex.
- Phenotypic and Genomic Divergence in Biofilm Formation in STEC and Non-STECPathogens (Basel, Switzerland) · 2026Article
- Characterizing the antibiotic resistance and virulence genes of diarrheagenic Escherichia coli from Brazilian beef cattle.BMC microbiology · 2026Article
- The Trade-Off Between Sanitizer Resistance and Virulence Genes: Genomic Insights intoAntibiotics (Basel, Switzerland) · 2025Article
- High intra-laboratory reproducibility of nanopore sequencing in bacterial species underscores advances in its accuracy.Microbial genomics · 2025Article
- Rapid, reference-free identification of bacterial pathogen transmission using optimized splitMicrobial genomics · 2025Article
- Article
- Evidence for Horizontal Transmission and Recirculation of Shiga Toxin-ProducingPathogens (Basel, Switzerland) · 2024Article
- Automating the Illumina DNA library preparation kit for whole genome sequencing applications on the flowbot ONE liquid handler robot.Scientific reports · 2024Article
- Pathogenomes of Shiga Toxin Positive and NegativeMicroorganisms · 2024Article
- High Genetic Diversity in Third-Generation Cephalosporin-ResistantPathogens (Basel, Switzerland) · 2024Article
- A preliminary study of the use of MinION sequencing to specifically detect Shiga toxin-producing Escherichia coli in culture swipes containing multiple serovars of this species.Scientific reports · 2023Article
- Phylogenetic relationship and virulence composition ofFrontiers in microbiology · 2023Article
- Genomic Analysis of Shiga Toxin-ProducingToxins · 2022Article
- Prevalence and Whole-Genome Sequence-Based Analysis of Shiga Toxin-Producing Escherichia coli Isolates from the Recto-Anal Junction of Slaughter-Age Irish Sheep.Applied and environmental microbiology · 2021Article
- Whole-Genome Sequencing Analysis of aDiagnostics (Basel, Switzerland) · 2021Article
- Prevalence and Epidemiology of Non-O157 Escherichia coli Serogroups O26, O103, O111, and O145 and Shiga Toxin Gene Carriage in Scottish Cattle, 2014-2015.Applied and environmental microbiology · 2021Article
- Added Value of Genomic Surveillance of Virulence Factors in Shiga Toxin-ProducingFrontiers in microbiology · 2021Article
- Characterization of Shiga Toxin 2a Encoding Bacteriophages Isolated From High-Virulent O145:H25 Shiga Toxin-ProducingFrontiers in microbiology · 2021Article
- Inconsistent PCR detection of Shiga toxin-producing Escherichia coli: Insights from whole genome sequence analyses.PloS one · 2021Article
- Investigation of the Causes of ShigatoxigenicMicroorganisms · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 4 institutions in 3 countries.
Funding
Abstract
The dynamics related to the loss of stx genes from Shiga toxin-producing Escherichia coli remain unclear. Current diagnostic procedures have shortcomings in the detection and identification of STEC. This is partly owing to the fact that stx genes may be lost during an infection or in the laboratory. The aim of the present study was to provide new insight into in vivo and in vitro stx loss in order to improve diagnostic procedures. Results from the study support the theory that loss of stx is a strain-related phenomenon and not induced by patient factors. It was observed that one strain could lose stx both in vivo and in vitro. Whole genome comparison of stx-positive and stx-negative isolates from the same patient revealed that different genomic rearrangements, such as complete or partial loss of the parent prophage, may be factors in the loss of stx. Of diagnostic interest, it was shown that patients can be co-infected with different E. coli pathotypes. Therefore, identification of eae-positive, but stx-negative isolates should not be interpreted as "Shiga toxin-lost" E. coli without further testing. Growth and recovery of STEC were supported by different selective agar media for different strains, arguing for inclusion of several media in STEC diagnostics.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.