Evidence map›Paper›PMID 30267004›Full record

ArticleScientific reports2018

Live Cell Imaging of Bioorthogonally Labelled Proteins Generated With a Single Pyrrolysine tRNA Gene.

Noa Aloush, Tomer Schvartz, Andres I König, Sarit Cohen, Eugene Brozgol, Benjamin Tam, Dikla Nachmias, Oshrit Ben-David, Yuval Garini, Natalie Elia and 1 more

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
1.7field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 28 citations in OpenAlex.

  1. Review
  2. Review
  3. Bioorthogonal Reactions in Bioimaging.Topics in current chemistry (Cham) · 2024
    Review
  4. Review
  5. Review
  6. Article
  7. Article
  8. Article
  9. Efficient Amber SuppressionACS synthetic biology · 2022
    Article
  10. Article
  11. An inside look at a biofilm:Science advances · 2021
    Article
  12. Review
  13. Article
  14. Expanding the Genetic Code for Neuronal Studies.Chembiochem : a European journal of chemical biology · 2020
    Review
  15. Site-Specific Protein Labeling with Fluorophores as a Tool To Monitor Protein Turnover.Chembiochem : a European journal of chemical biology · 2020
    Article
  16. Article
  17. Article
  18. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Noa AloushDepartment of Chemistry, Ben-Gurion University of the Negev, P.O. Box 653, Beer-Sheva, 8410501, Israel.
Tomer SchvartzDepartment of Life Sciences, Ben-Gurion University of the Negev, P.O. Box 653, Beer-Sheva, 8410501, Israel.
Andres I KönigDepartment of Life Sciences, Ben-Gurion University of the Negev, P.O. Box 653, Beer-Sheva, 8410501, Israel.
Sarit CohenDepartment of Chemistry, Ben-Gurion University of the Negev, P.O. Box 653, Beer-Sheva, 8410501, Israel.
Eugene BrozgolPhysics Department and Institute for Nanotechnology, Bar Ilan University, Ramat Gan, 5290002, Israel.
Benjamin TamDepartment of Chemistry, Ben-Gurion University of the Negev, P.O. Box 653, Beer-Sheva, 8410501, Israel.
Dikla NachmiasDepartment of Life Sciences, Ben-Gurion University of the Negev, P.O. Box 653, Beer-Sheva, 8410501, Israel.
Oshrit Ben-DavidDepartment of Chemistry, Ben-Gurion University of the Negev, P.O. Box 653, Beer-Sheva, 8410501, Israel.
Yuval GariniPhysics Department and Institute for Nanotechnology, Bar Ilan University, Ramat Gan, 5290002, Israel.
Natalie EliaDepartment of Life Sciences, Ben-Gurion University of the Negev, P.O. Box 653, Beer-Sheva, 8410501, Israel.ORCID http://orcid.org/0000-0002-2537-6173
Eyal ArbelyDepartment of Chemistry, Ben-Gurion University of the Negev, P.O. Box 653, Beer-Sheva, 8410501, Israel. arbely@bgu.ac.il.ORCID http://orcid.org/0000-0002-0284-0092
Ben-Gurion University of the Negev · ILBar-Ilan University · IL

Funding

DOD | Army Research Office (ARO) 65422-LSEC | European Research Council (ERC) 639313EC | European Research Council (ERC) 678461Israel Science Foundation (ISF) 807/15
6 · The paper itself

Abstract

Genetic code expansion enables the incorporation of non-canonical amino acids (ncAAs) into expressed proteins. ncAAs are usually encoded by a stop codon that is decoded by an exogenous orthogonal aminoacyl tRNA synthetase and its cognate suppressor tRNA, such as the pyrrolysine [Formula: see text] pair. In such systems, stop codon suppression is dependent on the intracellular levels of the exogenous tRNA. Therefore, multiple copies of the tRNA

Indexed as

Protein BiosynthesisAnimalsChlorocebus aethiopsCodon, TerminatorCOS CellsGenetic CodeLysineOptical ImagingProteinsRNA, TransferTransfectionCodon, TerminatorLysineProteinspyrrolysineRNA, Transfer

Identifiers

PMID30267004
PMCPMC6162220
OpenAlexW2891252361

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.