ArticleScientific reports2018
Antimicrobial genes from Allium sativum and Pinellia ternata revealed by a Bacillus subtilis expression system.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 36 citations in OpenAlex.
- A small antimicrobial peptide derived from a Burkholderia bacterium exhibits a broad-spectrum and high inhibiting activities against crop diseases.Plant biotechnology journal · 2025Article
- Transcriptomic and biochemical insights into key gene networks driving bulbil development of Pinellia ternata (Thunb.) Breit.PloS one · 2025Article
- Comparative transcriptome analysis reveals defense responses against soft rot induced by Pectobacterium aroidearum and Pectobacterium carotovorum in Pinellia ternata.BMC genomics · 2024Article
- Features of bacterial and fungal communities in the rhizosphere ofFrontiers in microbiology · 2024Article
- Transcriptome Profiling Reveals Differential Gene Expression during the Process of Microtuber Formation inInternational journal of molecular sciences · 2023Article
- Isolation and Optimization of a Broad-Spectrum Synthetic Antimicrobial Peptide, Ap920-WI, fromInternational journal of molecular sciences · 2023Article
- Integrated physicochemical, hormonal, and transcriptomic analysis reveals the underlying mechanism of callus formation inFrontiers in plant science · 2023Article
- Macrolactin A mediated biocontrol ofFrontiers in microbiology · 2023Article
- Isolation of Potato Endophytes and Screening ofInternational journal of molecular sciences · 2022Article
- Exploring the Potential Antidepressant Mechanisms of Pinellia by Using the Network Pharmacology and Molecular Docking.Metabolic brain disease · 2022Article
- A Novel Deoxynivalenol-Activated WheatJournal of fungi (Basel, Switzerland) · 2021Article
- Evaluation of qualitative traits in modified Iranian Red Rey onion and comparison genetic resistance with primary mass selection and Red Azar-shahr cv to Fusarium oxysporum using laboratory and molecular markers.Molecular biology reports · 2021Article
- Antimicrobial Peptides from Plants: A cDNA-Library Based Isolation, Purification, Characterization Approach and Elucidating Their Modes of Action.International journal of molecular sciences · 2021Review
- Prediction and Activity of a Cationic α-Helix Antimicrobial Peptide ZM-804 from Maize.International journal of molecular sciences · 2021Article
- Isolation of Antimicrobial Genes fromInternational journal of molecular sciences · 2020Article
- Two antimicrobial genes from Aegilops tauschii Cosson identified by the Bacillus subtilis expression system.Scientific reports · 2020Article
- Prediction and Characterization of Cationic Arginine-Rich Plant Antimicrobial Peptide SM-985 From Teosinte (Frontiers in microbiology · 2020Article
- Functional divergence of flagellar type III secretion system: A case study in a non-flagellated, predatory bacterium.Computational and structural biotechnology journal · 2020Article
- Cell Membrane-Interrupting Antimicrobial Peptides fromBiomolecules · 2019Article
- Enhanced Nicotiana benthamiana immune responses caused by heterologous plant genes from Pinellia ternata.BMC plant biology · 2018Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Antimicrobial genes are found in all classes of life. To efficiently isolate these genes, we used Bacillus subtilis and Escherichia coli as target indicator bacteria and transformed them with cDNA libraries. Among thousands of expressed proteins, candidate proteins played antimicrobial roles from the inside of the indicator bacteria (internal effect), contributing to the sensitivity (much more sensitivity than the external effect from antimicrobial proteins working from outside of the cells) and the high throughput ability of screening. We found that B. subtilis is more efficient and reliable than E. coli. Using the B. subtilis expression system, we identified 19 novel, broad-spectrum antimicrobial genes. Proteins expressed by these genes were extracted and tested, exhibiting strong external antibacterial, antifungal and nematicidal activities. Furthermore, these newly isolated proteins could control plant diseases. Application of these proteins secreted by engineered B. subtilis in soil could inhibit the growth of pathogenic bacteria. These proteins are thermally stable and suitable for clinical medicine, as they exhibited no haemolytic activity. Based on our findings, we speculated that plant, animal and human pathogenic bacteria, fungi or even cancer cells might be taken as the indicator target cells for screening specific resistance genes.
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