ArticleClinical and experimental immunology1986
Extraction of immune and inflammatory cells from human lung parenchyma: evaluation of an enzymatic digestion procedure.
Article in Clinical and experimental immunology, 1986. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 86 citations in OpenAlex.
- Transcriptome profiling in swine macrophages infected with African swine fever virus at single-cell resolution.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition).European journal of immunology · 2021Review
- An Optimized Method to Isolate Human Fibroblasts from Tissue forBio-protocol · 2019Article
- Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition).European journal of immunology · 2019Article
- Trigger-happy resident memory CD4Mucosal immunology · 2018Article
- Functional Heterogeneity of CD4Frontiers in immunology · 2018Article
- Programs for the persistence, vigilance and control of human CD8Nature immunology · 2016Article
- An optimized disaggregation method for human lung tumors that preserves the phenotype and function of the immune cells.Journal of leukocyte biology · 2015Article
- Smoking enhances the proinflammatory effects of nucleotides on cytokine release from human lung.PloS one · 2014Article
- Impact of enzymatic tissue disintegration on the level of surface molecule expression and immune cell function.European journal of microbiology & immunology · 2012Article
- CD8⁺ T cells with an intraepithelial phenotype upregulate cytotoxic function upon influenza infection in human lung.The Journal of clinical investigation · 2011Article
- Resident memory T cells (T(RM)) are abundant in human lung: diversity, function, and antigen specificity.PloS one · 2011Article
- Selective accumulation of differentiated CD8+ T cells specific for respiratory viruses in the human lung.The Journal of experimental medicine · 2005Article
- Characterization of T cell clones derived from lymph nodes and lungs of Pseudomonas aeruginosa-susceptible and resistant mice following immunization with heat-killed bacteria.Clinical and experimental immunology · 2000Article
- Immunophenotyping of bronchoalveolar lavage lymphocytes.Clinical and diagnostic laboratory immunology · 1998Review
- Accumulation of activated CD4+ lymphocytes in the lung of individuals infected with HIV accompanied by increased virus production in patients with secondary infections.Clinical and experimental immunology · 1995Article
- Lymphocytes in the lung: an often neglected cell. Numbers, characterization and compartmentalization.Anatomy and embryology · 1995Review
- The regulation of pulmonary immunity.Advances in immunology · 1995Review
- In vitro and in vivo T cell responses in mice during bronchopulmonary infection with mucoid Pseudomonas aeruginosa.Clinical and experimental immunology · 1995Article
- The T cell antigen receptor CD3:CD4 molecular complex is diminished on the surface of pulmonary lymphocytes.The American journal of pathology · 1994Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The inflammatory and immune cell populations of the human lung parenchyma have not been characterized in detail. This report describes a novel and efficient procedure for their extraction. Histologically normal human lung tissue samples from pneumonectomy specimens were sliced to 0.5 mm, and digested in collagenase/DNAse. Viable mononuclear cell yields ranged from 15-48 X 10(6)/g, and were markedly in excess of reported methods employing mechanical tissue disruption, which normally yield populations containing almost exclusively macrophages. The lung digest population was examined by flow cytometry using monoclonal antibodies against cell surface receptors, and found to comprise up to 40% T lymphocytes, 10% B lymphocytes and 30% macrophages, contaminated by less than 1% peripheral blood cells. Based upon these figures, the recoverable lung parenchymal lymphoid cell pool appears considerably larger than previously recognized, being of the same order as the peripheral blood pool. Initial functional studies suggest that such cellular activities as antigen-specific T cell proliferation, antigen-presentation, interleukin 1 production and natural killer cell activity survive the extraction process, and controlled enzymatic digestion experiments with peripheral blood cells indicate that the degree of enzyme-mediated damage to these functions and to cell-surface structures, was minimal. The extraction method thus appears suitable for studying the types and functions of human parenchymal lung cells in health and disease.
Indexed as
Identifiers
3026698PMC1542646W34244713What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.