ArticleDiagnostic microbiology and infectious disease2019
A highly sensitive 1-tube nested real-time RT-PCR assay using LNA-modified primers for detection of respiratory syncytial virus.
Article in Diagnostic microbiology and infectious disease, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- A Nested PCR-Based Point of Care Testing of Multiplex Pathogens Associated with Bloodstream Infection.Pathogens (Basel, Switzerland) · 2026Article
- A nested PCR-based point-of-care multiplex test for detection of bacterial pathogens in cerebrospinal fluids.Frontiers in cellular and infection microbiology · 2026Article
- Participant perceptions of the impact of the ModPad program on molecular diagnostic capacity building and public health practices.Frontiers in public health · 2026Article
- Evaluation of common respiratory viruses other than SARS-CoV-2 in hospitalized children during the COVID-19 pandemic.BMC infectious diseases · 2025Article
- Rapid detection of human adenovirus subgroup B using recombinase polymerase amplification assay.Virus genes · 2024Article
- Development of Rapid and Visual Nucleic Acid Detection Methods towards Four Serotypes of Human Adenovirus Species B Based on RPA-LF Test.BioMed research international · 2021Article
- Recent advances in the detection of respiratory virus infection in humans.Journal of medical virology · 2020Review
- One-Tube Nested Real-Time PCR Assay for Rapid Screening of Porcine Cytomegalovirus in Clinical Samples.Frontiers in veterinary science · 2020Article
- Development and evaluation of recombinase-aided amplification assays incorporating competitive internal controls for detection of human adenovirus serotypes 3 and 7.Virology journal · 2019Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Respiratory syncytial virus (RSV) causes serious respiratory tract infection worldwide. The relatively low RSV load makes it difficult to detect in frail, elderly, and severely immune-compromised patients. In the present study, we developed a locked nucleic acid--based 1-tube nested real-time RT-PCR (OTNRT-PCR) assay with the advantages of extremely high sensitivity, facile operability, and less likelihood of cross-contamination. The sensitivity, specificity, and clinical performance of the OTNRT-PCR assay were compared in parallel with a conventional TaqMan probe-based real-time PCR (qRT-PCR) assay and a traditional 2-step nested RT-PCR assay. The limit of detection of the OTNRT-PCR assay was 1.02 × 10
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