ArticleiScience2018
Live-Cell Imaging and Functional Dissection of Xist RNA Reveal Mechanisms of X Chromosome Inactivation and Reactivation.
Article in iScience, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 23 citations in OpenAlex.
- Real-time single-molecule imaging of transcriptional regulatory networks in living cells.Nature reviews. Genetics · 2024Review
- Early chromosome condensation by XIST builds A-repeat RNA density that facilitates gene silencing.Cell reports · 2023Article
- Live cell imaging of DNA and RNA with fluorescent signal amplification and background reduction techniques.Frontiers in cell and developmental biology · 2023Review
- Gene regulation in time and space during X-chromosome inactivation.Nature reviews. Molecular cell biology · 2022Review
- The lupus autoantigen La/Ssb is an Xist-binding protein involved in Xist folding and cloud formation.Nucleic acids research · 2021Article
- The control of polycomb repressive complexes by long noncoding RNAs.Wiley interdisciplinary reviews. RNA · 2021Review
- Elements at the 5' end of Xist harbor SPEN-independent transcriptional antiterminator activity.Nucleic acids research · 2020Article
- Xist Repeats A and B Account for Two Distinct Phases of X Inactivation Establishment.Developmental cell · 2020Article
- Imaging of DNA and RNA in Living Eukaryotic Cells to Reveal Spatiotemporal Dynamics of Gene Expression.Annual review of biochemistry · 2020Review
- How to silence an X chromosome.Nature · 2020Article
- RETRACTED: A 5' fragment of Xist can sequester RNA produced from adjacent genes on chromatin.Nucleic acids research · 2019Article
- Recent Advances in Understanding the Reversal of Gene Silencing During X Chromosome Reactivation.Frontiers in cell and developmental biology · 2019Review
- Beyond classic editing: innovative CRISPR approaches for functional studies of long non-coding RNA.Biology methods & protocols · 2019Review
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We double-tagged Xist (inactivated X chromosome-specific transcript), a prototype long non-coding RNA pivotal for X chromosome inactivation (XCI), using the programmable RNA sequence binding domain of Pumilio protein, one tag for live-cell imaging and the other replacing A-repeat (a critical domain of Xist) to generate "ΔA mutant" and to tether effector proteins for dissecting Xist functionality. Based on the observation in live cells that the induced XCI in undifferentiated embryonic stem (ES) cells is counteracted by the intrinsic X chromosome reactivation (XCR), we identified Kat8 and Msl2, homologs of Drosophila dosage compensation proteins, as players involved in mammalian XCR. Furthermore, live-cell imaging revealed the obviously undersized ΔA Xist cloud signals, clarifying an issue regarding the previous RNA fluorescence in situ hybridization results. Tethering candidate proteins onto the ΔA mutant reveals the significant roles of Ythdc1, Ezh2, and SPOC (Spen) in Xist-mediated gene silencing and the significant role of Ezh2 in Xist RNA spreading.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.