ArticleGenome biology and evolution2018
A Single Tim Translocase in the Mitosomes of Giardia intestinalis Illustrates Convergence of Protein Import Machines in Anaerobic Eukaryotes.
Article in Genome biology and evolution, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 52 citations in OpenAlex.
- Extreme mitochondrial reduction in a novel group of free-living metamonads.Nature communications · 2024Article
- Pam16 and Pam18 were repurposed during Trypanosoma brucei evolution to regulate the replication of mitochondrial DNA.PLoS biology · 2024Article
- A hybrid TIM complex mediates protein import into hydrogenosomes of Trichomonas vaginalis.BMC biology · 2024Article
- Genomics of Preaxostyla Flagellates Illuminates the Path Towards the Loss of Mitochondria.PLoS genetics · 2023Article
- Lessons from the deep: mechanisms behind diversification of eukaryotic protein complexes.Biological reviews of the Cambridge Philosophical Society · 2023Article
- Molecular pathway of mitochondrial preprotein import through the TOM-TIM23 supercomplex.Nature structural & molecular biology · 2023Article
- Adaptation of the late ISC pathway in the anaerobic mitochondrial organelles of Giardia intestinalis.PLoS pathogens · 2023Article
- Article
- Reduced mitochondria provide an essential function for the cytosolic methionine cycle.Current biology : CB · 2022Article
- Evidence for an Independent Hydrogenosome-to-Mitosome Transition in the CL3 Lineage of Fornicates.Frontiers in microbiology · 2022Article
- High quality genome assembly of the amitochondriate eukaryoteMicrobial genomics · 2021Article
- Inheritance of the reduced mitochondria of Giardia intestinalis is coupled to the flagellar maturation cycle.BMC biology · 2021Article
- Retortamonads from vertebrate hosts share features of anaerobic metabolism and pre-adaptations to parasitism with diplomonads.Parasitology international · 2021Article
- The Mastigamoeba balamuthi Genome and the Nature of the Free-Living Ancestor of Entamoeba.Molecular biology and evolution · 2021Article
- Analysis of diverse eukaryotes suggests the existence of an ancestral mitochondrial apparatus derived from the bacterial type II secretion system.Nature communications · 2021Article
- Novel IM-associated protein Tim54 plays a role in the mitochondrial import of internal signal-containing proteins in Trypanosoma brucei.Biology of the cell · 2021Article
- Tim17 Updates: A Comprehensive Review of an Ancient Mitochondrial Protein Translocator.Biomolecules · 2020Review
- tRNA import across the mitochondrial inner membrane in T. brucei requires TIM subunits but is independent of protein import.Nucleic acids research · 2020Article
- Import ofMicroorganisms · 2020Article
- Article
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
Mitochondria have evolved diverse forms across eukaryotic diversity in adaptation to anoxia. Mitosomes are the simplest and the least well-studied type of anaerobic mitochondria. Transport of proteins via TIM complexes, composed of three proteins of the Tim17 protein family (Tim17/22/23), is one of the key unifying aspects of mitochondria and mitochondria-derived organelles. However, multiple experimental and bioinformatic attempts have so far failed to identify the nature of TIM in mitosomes of the anaerobic metamonad protist, Giardia intestinalis, one of the few experimental models for mitosome biology. Here, we present the identification of a single G. intestinalis Tim17 protein (GiTim17), made possible only by the implementation of a metamonad-specific hidden Markov model. While very divergent in primary sequence and in predicted membrane topology, experimental data suggest that GiTim17 is an inner membrane mitosomal protein, forming a disulphide-linked dimer. We suggest that the peculiar GiTim17 sequence reflects adaptation to the unusual, detergent resistant, inner mitosomal membrane. Specific pull-down experiments indicate interaction of GiTim17 with mitosomal Tim44, the tethering component of the import motor complex. Analysis of TIM complexes across eukaryote diversity suggests that a "single Tim" translocase is a convergent adaptation of mitosomes in anaerobic protists, with Tim22 and Tim17 (but not Tim23), providing the protein backbone.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.