Evidence map›Paper›PMID 30264926›Full record

ArticleInfluenza and other respiratory viruses2019

A newly developed tetraplex real-time RT-PCR for simultaneous screening of influenza virus types A, B, C and D.

Dinah Henritzi, Bernd Hoffmann, Silke Wacheck, Stefan Pesch, Georg Herrler, Martin Beer, Timm C Harder

Open access · goldAbstract read
In one paragraph

Article in Influenza and other respiratory viruses, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed, 2 pooled it
2.6field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 2 syntheses or guidelines pooled it, 49 citations in OpenAlex.

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  6. Monitoring for respiratory viruses among wild canids, Texas.One health (Amsterdam, Netherlands) · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Dinah HenritziInstitute of Diagnostic Virology, Friedrich-Loeffler-Institut (FLI), Greifswald-Insel Riems, Germany.ORCID 0000-0002-2880-8795
Bernd HoffmannInstitute of Diagnostic Virology, Friedrich-Loeffler-Institut (FLI), Greifswald-Insel Riems, Germany.
Silke WacheckIDT Biologika GmbH, Dessau-Rosslau, Germany.
Stefan PeschIDT Biologika GmbH, Dessau-Rosslau, Germany.
Georg HerrlerUniversity of Veterinary Medicine Hannover, Foundation, Hannover, Germany.
Martin BeerInstitute of Diagnostic Virology, Friedrich-Loeffler-Institut (FLI), Greifswald-Insel Riems, Germany.
Timm C HarderInstitute of Diagnostic Virology, Friedrich-Loeffler-Institut (FLI), Greifswald-Insel Riems, Germany.
Friedrich-Loeffler-Institut · DEIDT Biologika (Germany) · DEUniversity of Veterinary Medicine Hannover, Foundation · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundHuman- or avian-to-swine transmissions have founded several autonomously circulating influenza A virus (IAV) lineages in swine populations that cause economically important respiratory disease. Little is known on other human influenza virus types, like B (IBV) and C (ICV) in European swine, and of the recently detected novel animal influenza virus type D (IDV).

objectivesDevelopment of a cost-effective diagnostic tool for large-scale surveillance programmes targeting all four influenza virus types.

methodsAn influenza ABCD tetraplex real-time RT-PCR (RT-qPCR) was developed in the frame of this study. A selection of reference virus strains and more than 4000 porcine samples from a passive IAV surveillance programme in European swine with acute respiratory disease were examined.

resultsTwo IBV, a single IDV but no ICV infections were identified by tetraplex RT-qPCR. IBV and IDV results were confirmed by conventional RT-PCR and partial sequence analysis.

conclusionsThe tetraplex RT-qPCR proved fit for purpose as a sensitive, specific and high-throughput tool to study influenza virus transmission at the human-animal interface. Complementing close-meshed active virological and serological surveillance is required to better understand the true incidence and prevalence of influenza virus type B, C and D infections in swine.

Indexed as

AnimalsDNA PrimersEpidemiological MonitoringEuropeGammainfluenzavirusHigh-Throughput Screening AssaysInfluenza A virusInfluenza B virusMultiplex Polymerase Chain ReactionOrthomyxoviridaeOrthomyxoviridae InfectionsRNA, ViralSensitivity and SpecificitySus scrofaSwineSwine DiseasesDNA PrimersRNA, ViralEuropean surveillanceinfluenza virus types A, B, C and Dmultiplex RT-qPCRswine

Identifiers

PMID30264926
PMCPMC6304318
OpenAlexW2893833068

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.