Evidence map›Paper›PMID 30264882›Full record

ArticleXenotransplantation2018

Xenotransplantation panel for the detection of infectious agents in pigs.

Caroll B Hartline, Ra'Shun L Conner, Scott H James, Jennifer Potter, Edward Gray, Jose Estrada, Mathew Tector, A Joseph Tector, Mark N Prichard

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Xenotransplantation, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
4.7field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 36 citations in OpenAlex.

  1. Review
  2. Infectious Diseases and Clinical Xenotransplantation.Emerging infectious diseases · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Caroll B HartlineDepartment of Pediatrics, University of Alabama School of Medicine, Birmingham, AL, USA.
Ra'Shun L ConnerDepartment of Pediatrics, University of Alabama School of Medicine, Birmingham, AL, USA.
Scott H JamesDepartment of Pediatrics, University of Alabama School of Medicine, Birmingham, AL, USA.
Jennifer PotterDepartment of Pediatrics, University of Alabama School of Medicine, Birmingham, AL, USA.
Edward GrayDepartment of Surgery, University of Alabama School of Medicine, Birmingham, AL, USA.
Jose EstradaDepartment of Surgery, University of Alabama School of Medicine, Birmingham, AL, USA.
Mathew TectorDepartment of Surgery, University of Alabama School of Medicine, Birmingham, AL, USA.
A Joseph TectorDepartment of Surgery, University of Alabama School of Medicine, Birmingham, AL, USA.
Mark N PrichardDepartment of Pediatrics, University of Alabama School of Medicine, Birmingham, AL, USA.ORCID 0000-0002-2720-6661
University of Alabama · USUniversity of Alabama at Birmingham · US

Funding

Optimizing Strategies to Overcome Kidney Xenograft RejectionR01AI126322 · NIAID · UNIVERSITY OF MINNESOTA · PI ADAMS, ANDREW B · 2016 to 2020
$4.6M
NIAID NIH HHS R01 AI126322NIH HHS 5R01AI126322-02UAB Department of Pediatrics (MNP)
6 · The paper itself

Abstract

backgroundRecent advances in xenotransplantation have produced organs from pigs that are well tolerated in primate models because of genetic changes engineered to delete major antigens from donor animals. To ensure the safety of human transplant recipients, it will be essential to understand both the spectrum of infectious agents in donor pigs and their potential to be transmitted to immunocompromised transplant recipients. Equally important will be the development of new highly sensitive diagnostic methods for use in the detection of these agents in donor animals and for the monitoring of transplant recipients.

methodsHerein, we report the development of a panel of 30 quantitative polymerase chain reaction (qPCR) assays for infectious agents with the potential to be transmitted to the human host. The reproducibility, sensitivity and specificity of each assay were evaluated and were found to exhibit analytic sensitivity that was similar to that of quantitative assays used to perform viral load testing of human viruses in clinical laboratories.

resultsThis analytical approach was used to detect nucleic acids of infectious agents present in specimens from 9 sows and 22 piglets derived by caesarean section. The most commonly detected targets in adult animals were Mycoplasma species and two distinct herpesviruses, porcine lymphotrophic herpesvirus 2 and 3. A total of 14 piglets were derived from three sows infected with either or both herpesviruses, yet none tested positive for the viruses indicating that vertical transmission of these viruses is inefficient.

conclusionsThe data presented demonstrate that procedures in place are highly sensitive and can specifically detect nucleic acids from target organisms in the panel, thus ensuring the safety of organs for transplantation as well as the monitoring of patients potentially receiving them.

Indexed as

AnimalsCytomegalovirusHerpesviridaeHeterograftsHumansReproducibility of ResultsSwineSwine DiseasesTransplantation, HeterologouscoronavirusesherpesvirusesInfectious disease panelvirus safety of xenotransplantation

Identifiers

PMID30264882
PMCPMC6166664
OpenAlexW2885299834

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.