ArticleScience advances2018
Evaluating biological activity of compounds by transcription factor activity profiling.
Article in Science advances, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed.
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- Burnt Plastic (Pyroplastic) from the M/VEnvironment & health (Washington, D.C.) · 2025Article
- Humin oxidation drives microbial dehalogenation in oligotrophic environments.The ISME journal · 2025Article
- Biological functions and molecular mechanisms of MORC2 in human diseases.Molecules and cells · 2025Review
- Engineering a computable epiblast for in silico modeling of developmental toxicity.Reproductive toxicology (Elmsford, N.Y.) · 2024Article
- Moldable Plastics (Polycaprolactone) can be Acutely Toxic to Developing Zebrafish and Activate Nuclear Receptors in Mammalian Cells.ACS biomaterials science & engineering · 2024Article
- A 2024 inventory of test methods relevant to thyroid hormone system disruption for human health and environmental regulatory hazard assessment.Open research Europe · 2024Review
- Evaluation of Per- and Polyfluoroalkyl Substances (PFAS)Chemical research in toxicology · 2023Article
- Regulome-based characterization of drug activity across the human diseasome.NPJ systems biology and applications · 2022Article
- Advances in computational methods along the exposure to toxicological response paradigm.Toxicology and applied pharmacology · 2022Article
- PCR-based profiling of transcription factor activityiScience · 2022Article
- A novel chemo-phenotypic method identifies mixtures of salpn, vitamin D3, and pesticides involved in the development of colorectal and pancreatic cancer.Ecotoxicology and environmental safety · 2022Article
- Comprehensive assessment of NR ligand polypharmacology by a multiplex reporter NR assay.Scientific reports · 2022Article
- Estimating Hepatotoxic Doses Using High-Content Imaging in Primary Hepatocytes.Toxicological sciences : an official journal of the Society of Toxicology · 2021Article
- Evaluation of a multiplexed, multispecies nuclear receptor assay for chemical hazard assessment.Toxicology in vitro : an international journal published in association with BIBRA · 2021Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Assessing the biological activity of compounds is an essential objective of biomedical research. We show that one can infer the bioactivity of compounds by assessing the activity of transcription factors (TFs) that regulate gene expression. Using a multiplex reporter system, the FACTORIAL, we characterized cell response to a compound by a quantitative signature, the TF activity profile (TFAP). We found that perturbagens of biological pathways elicited distinct TFAP signatures in human cells. Unexpectedly, perturbagens of the same pathway all produced identical TFAPs, regardless of where or how they interfered. We found invariant TFAPs for mitochondrial, histone deacetylase, and ubiquitin/proteasome pathway inhibitors; cytoskeleton disruptors; and DNA-damaging agents. Using these invariant signatures permitted straightforward identification of compounds with specified bioactivities among uncharacterized chemicals. Furthermore, this approach allowed us to assess the multiple bioactivities of polypharmacological drugs. Thus, TF activity profiling affords straightforward assessment of the bioactivity of compounds through the identification of perturbed biological pathways.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.