ArticleeNeuro
High Fidelity Cryopreservation and Recovery of Primary Rodent Cortical Neurons.
Article in eNeuro. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The trial behind it
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Who cites it
17 citing papers in PubMed.
- An improved preservation method for human dorsal root ganglion neurons enables wider access to human molecular pain neuroscience.Cell reports methods · 2026Article
- Enabling wider access to human molecular neuroscience research in pain: A simple preservation method for human dorsal root ganglion neurons in Hibernate A media.bioRxiv : the preprint server for biology · 2025Article
- Method for cryopreservation of brainstem pons and medulla oblongata tissue from Sprague Dawley rats for establishing primary mixed neuron-glia cell cultures.Brain research · 2025Article
- Defining neuronal responses to the neurotropic parasitemSphere · 2025Article
- Ref-1 redox activity modulates canonical Wnt signaling in endothelial cells.Redox biology · 2025Article
- Cryopreserving 3D cell culture models of Alzheimer's disease in hydrogel microbeads.Scientific reports · 2025Article
- N-Cadherin based adhesion and Rac1 activity regulate tension polarization in the actin cortex.Scientific reports · 2025Article
- Tankyrase inhibition interferes with junction remodeling, induces leakiness, and disturbs YAP1/TAZ signaling in the endothelium.Naunyn-Schmiedeberg's archives of pharmacology · 2024Article
- Method for cryopreservation of trigeminal ganglion for establishing primary cultures of neurons and glia.Journal of neuroscience methods · 2024Article
- EVL and MIM/MTSS1 regulate actin cytoskeletal remodeling to promote dendritic filopodia in neurons.The Journal of cell biology · 2023Article
- ROP16-mediated activation of STAT6 enhances cyst development of type III Toxoplasma gondii in neurons.PLoS pathogens · 2023Article
- IFN-γ stimulated murine and human neurons mount anti-parasitic defenses against the intracellular parasite Toxoplasma gondii.Nature communications · 2022Article
- Hyperexcitability and Pharmacological Responsiveness of Cortical Neurons Derived from Human iPSCs Carrying Epilepsy-Associated Sodium Channel Nav1.2-L1342P Genetic Variant.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Neuronal cell-based medicines from pluripotent stem cells: Development, production, and preclinical assessment.Stem cells translational medicine · 2021Review
- Biophysical matrix cues from the regenerating niche direct muscle stem cell fate in engineered microenvironments.Biomaterials · 2021Article
- Human serum albumin and chromatin condensation rescue ex vivo expanded γδ T cells from the effects of cryopreservation.Cryobiology · 2021Article
- Differentiation of Human iPS Cells Into Sensory Neurons Exhibits Developmental Stage-Specific Cryopreservation Challenges.Frontiers in cell and developmental biology · 2021Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Cell cryopreservation improves reproducibility and enables flexibility in experimental design. Although conventional freezing methodologies have been used to preserve primary neurons, poor cell viability and reduced survival severely limited their utility. We screened several high-performance freezing media and found that CryoStor10 (CS10) provided superior cryoprotection to primary mouse embryonic cortical neurons compared to other commercially-available or traditional reagents, permitting the recovery of 68.8% of cells relative to a fresh dissection. We characterized developmental, morphometric, and functional indicators of neuron maturation and found that, without exception, neurons recovered from cryostorage in CS10 media faithfully recapitulate
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.