ArticleScientific reports2018
A single reporter mouse line for Vika, Flp, Dre, and Cre-recombination.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 29 citations in OpenAlex.
- WTR: A Toolkit for Functional Anterograde Transsynaptic Circuit Mapping.Research square · 2026Article
- Advances in large-scale DNA engineering with the CRISPR system.Experimental & molecular medicine · 2025Review
- A Strictly Inducible and Orthogonal Dre-rox System for Precise and Markerless Genome Editing inJournal of microbiology and biotechnology · 2025Article
- One-step Cre-loxP organism creation by TAx9.Communications biology · 2025Article
- Multistep allelic conversion in mouse pre-implantation embryos by AAV vectors.Scientific reports · 2024Article
- Unlocking Genetic Mysteries during the Epic Sperm Journey toward Fertilization: Further ExpandingBiomolecules · 2024Review
- Review
- Nomenclature for standardized designation of diploid genotypes in genetically modified laboratory animals.Laboratory animals · 2023Article
- Discovery and characterization of novel Cre-type tyrosine site-specific recombinases for advanced genome engineering.Nucleic acids research · 2023Article
- Proteolytic processing of galectin-3 by meprin metalloproteases is crucial for host-microbiome homeostasis.Science advances · 2023Article
- Construction and application of a multifunctional CHO cell platform utilizing Cre/Frontiers in bioengineering and biotechnology · 2023Article
- Targeted single-cell gene induction by optimizing the dually regulated CRE/Frontiers in plant science · 2023Article
- Differential CRH expression level determines efficiency of Cre- and Flp-dependent recombination.Frontiers in neuroscience · 2023Article
- A most formidable arsenal: genetic technologies for building a better mouse.Genes & development · 2020Review
- Article
- Neurobiology of cannabinoid receptor signaling .Dialogues in clinical neuroscience · 2020Review
- Nearest-neighbor amino acids of specificity-determining residues influence the activity of engineered Cre-type recombinases.Scientific reports · 2020Article
- Thy1 promoter activity in the Rosa26 locus in mice: lessons from Dre-rox conditional expression system.Experimental animals · 2020Article
- Principles of Genetic Engineering.Genes · 2020Review
- Parenting - a paradigm for investigating the neural circuit basis of behavior.Current opinion in neurobiology · 2020Review
Corrections and comments
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Authors and funding
6 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Site-specific recombinases (SSR) are utilized as important genome engineering tools to precisely modify the genome of mice and other model organisms. Reporter mice that mark cells that at any given time had expressed the enzyme are frequently used for lineage tracing and to characterize newly generated mice expressing a recombinase from a chosen promoter. With increasing sophistication of genome alteration strategies, the demand for novel SSR systems that efficiently and specifically recombine their targets is rising and several SSR-systems are now used in combination to address complex biological questions in vivo. Generation of reporter mice for each one of these recombinases is cumbersome and increases the number of mouse lines that need to be maintained in animal facilities. Here we present a multi-reporter mouse line for loci-of-recombination (X) (MuX) that streamlines the characterization of mice expressing prominent recombinases. MuX mice constitutively express nuclear green fluorescent protein after recombination by either Cre, Flp, Dre or Vika recombinase, rationalizing the number of animal lines that need to be maintained. We also pioneer the use of the Vika/vox system in mice, illustrating its high efficacy and specificity, thereby facilitating future designs of sophisticated recombinase-based in vivo genome engineering strategies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.