Evidence map›Paper›PMID 30262897›Full record

ArticleScientific reports2018

Bioprocessing strategies to enhance the challenging isolation of neuro-regenerative cells from olfactory mucosa.

Melanie Georgiou, Joana Neves Dos Reis, Rachael Wood, Patricia Perez Esteban, Victoria Roberton, Chris Mason, Daqing Li, Ying Li, David Choi, Ivan Wall

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.2field-weighted citation impact, top 52% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 2 countries.

Melanie GeorgiouDepartment of Biochemical Engineering, University College London, Torrington Place, London, WC1E 7JE, UK.
Joana Neves Dos ReisDepartment of Biochemical Engineering, University College London, Torrington Place, London, WC1E 7JE, UK.
Rachael WoodDepartment of Biochemical Engineering, University College London, Torrington Place, London, WC1E 7JE, UK.
Patricia Perez EstebanDepartment of Biochemical Engineering, University College London, Torrington Place, London, WC1E 7JE, UK.ORCID http://orcid.org/0000-0001-7084-1065
Victoria RobertonDepartment of Biochemical Engineering, University College London, Torrington Place, London, WC1E 7JE, UK.ORCID http://orcid.org/0000-0002-0404-6984
Chris MasonDepartment of Biochemical Engineering, University College London, Torrington Place, London, WC1E 7JE, UK.
Daqing LiSpinal Repair Unit, Department of Brain, Repair and Rehabilitation, Institute of Neurology, University College London, Queen Square, London, WC1N 3BG, UK.
Ying LiSpinal Repair Unit, Department of Brain, Repair and Rehabilitation, Institute of Neurology, University College London, Queen Square, London, WC1N 3BG, UK.
David ChoiSpinal Repair Unit, Department of Brain, Repair and Rehabilitation, Institute of Neurology, University College London, Queen Square, London, WC1N 3BG, UK.
Ivan WallDepartment of Biochemical Engineering, University College London, Torrington Place, London, WC1E 7JE, UK. i.wall@aston.ac.uk.
Aston University · GBNational Hospital for Neurology and Neurosurgery · GBUniversity College London · GBCell Therapy Catapult · GB

Funding

Biotechnology and Biological Sciences Research Council BB/K011154/1
6 · The paper itself

Abstract

Olfactory ensheathing cells (OECs) are a promising potential cell therapy to aid regeneration. However, there are significant challenges in isolating and characterizing them. In the current study, we have explored methods to enhance the recovery of cells expressing OEC marker p75NTR from rat mucosa. With the addition of a 24-hour differential adhesion step, the expression of p75NTR was significantly increased to 73 ± 5% and 46 ± 18% on PDL and laminin matrices respectively. Additionally, the introduction of neurotrophic factor NT-3 and the decrease in serum concentration to 2% FBS resulted in enrichment of OECs, with p75NTR at nearly 100% (100 ± 0% and 98 ± 2% on PDL and laminin respectively), and candidate fibroblast marker Thy1.1 decreased to zero. Culturing OECs at physiologically relevant oxygen tension (2-8%) had a negative impact on p75NTR expression and overall cell survival. Regarding cell potency, co-culture of OECs with NG108-15 neurons resulted in more neuronal growth and potential migration at atmospheric oxygen. Moreover, OECs behaved similarly to a Schwann cell line positive control. In conclusion, this work identified key bioprocessing fundamentals that will underpin future development of OEC-based cell therapies for potential use in spinal cord injury repair. However, there is still much work to do to create optimized isolation methods.

Indexed as

AnimalsCell Culture TechniquesCell LineCell SeparationNerve Growth FactorsNerve Tissue ProteinsNeuronsOlfactory MucosaRatsRats, Sprague-DawleyReceptors, Growth FactorReceptors, Nerve Growth FactorNerve Growth FactorsNerve Tissue ProteinsNgfr protein, ratReceptors, Growth FactorReceptors, Nerve Growth Factor

Identifiers

PMID30262897
PMCPMC6160430
OpenAlexW2891759763

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.