ArticleJournal of genetics2018
A minimum requirements method to isolate large quantities of highly purified DNA from one drop of poultry blood.
Article in Journal of genetics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Association between KISS1 gene and growth traits in two Middle Eastern sheep breeds: the Iranian Karakul and the Iraqi Awassi.Tropical animal health and production · 2025Article
- Comparing feed efficiency and egg production in Hy-Line W-80 and Iraqi indigenous chickens.Veterinary research forum : an international quarterly journal · 2025Article
- A Novel Missense SNP in the Fatty Acid-Binding Protein 4 (FABP4) Gene is Associated with Growth Traits in Karakul and Awassi Sheep.Biochemical genetics · 2024Article
- A novel intron variant in the prolactin gene associated with eggshell weight and thickness with putative alternative splicing patterns in chickens.Journal of genetics · 2024Article
- Two co-inherited SNPs of the telomerase reverse transcriptase (TERT) gene are associated with Iraqi patients with lung cancer.Journal of medical biochemistry · 2023Article
- Two missense variants of the epidermal growth factor receptor gene are associated with non small cell lung carcinoma in the subjects from Iraq.Molecular biology reports · 2022Article
- Detection of a novel single nucleotide polymorphism in IGF2 gene with a negative impact on egg production and body weight in Japanese quail (Coturnix japonica).Journal, genetic engineering & biotechnology · 2021Article
- DNA Extraction with TRIzol Reagent Using a Silica Column.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2021Article
- New Perspectives on Avian Models for Studies of Basic Aging Processes.Biomedicines · 2021Review
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1 author.
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Abstract
Here, we describe a rapid, efficient and noncomplicated method for extracting poultry genomic DNA. This simple method obtains excellent qualities of molecular biology grade DNA in a matter of only about 15 min. A straightforward protocol is followed in this extraction procedure, in which, when the blood cells are placed in a distilled water alone, water rapidly disrupts red blood cells (RBCs) membranes and alleviate chickens' undesired high blood viscosity without being aided by any other chemicals. Moreover, the time, cost and efforts were hugely reduced since the step of leukocytes lysis was successfully mixed with the protein precipitation. The isolated genomic DNA, in terms of its quantity and quality, is satisfying and is proved to be suitable for restriction endonucleases digestion, polymerase chain reaction (PCR), and restriction fragment length polymorphism (RFLP). Thus, instead of extracting a limited amount of DNA using expensive or relatively expensive kits, this guaranteed procedure can be utilized alternatively to accurately extract genomic DNA (gDNA) from minimal starter quantities of the chicken's blood that do not exceed 50
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