Evidence map›Paper›PMID 30262218›Full record

ArticleDifferentiation; research in biological diversity

Three-dimensional imaging of the developing human fetal urogenital-genital tract: Indifferent stage to male and female differentiation.

Dylan Isaacson, Joel Shen, Maya Overland, Yi Li, Adriane Sinclair, Mei Cao, Dylan McCreedy, Meredith Calvert, Todd McDevitt, Gerald R Cunha and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Differentiation; research in biological diversity. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
  2. Development of the human penis and clitoris.Differentiation; research in biological diversity
    Article
  3. Article
  4. Human glans and preputial development.Differentiation; research in biological diversity
    Article
  5. Article
  6. Penile Length can be Estimated by the Foot-Length? Study in Human Fetuses with Neural Tube Defects.International braz j urol : official journal of the Brazilian Society of Urology
    Article
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Dylan IsaacsonDepartment of Urology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.
Joel ShenDepartment of Urology, University of California, San Francisco, San Francisco, CA, USA.
Maya OverlandDepartment of Urology, University of California, San Francisco, San Francisco, CA, USA.
Yi LiDepartment of Urology, University of California, San Francisco, San Francisco, CA, USA.
Adriane SinclairDepartment of Urology, University of California, San Francisco, San Francisco, CA, USA.
Mei CaoDepartment of Urology, University of California, San Francisco, San Francisco, CA, USA.
Dylan McCreedyJ. David Gladstone Institutes, San Francisco, CA, USA.
Meredith CalvertJ. David Gladstone Institutes, San Francisco, CA, USA; Histology and Light Microscopy Core, J. David Gladstone Institutes, San Francisco, CA, USA.
Todd McDevittJ. David Gladstone Institutes, San Francisco, CA, USA.
Gerald R CunhaDepartment of Urology, University of California, San Francisco, San Francisco, CA, USA.
Laurence BaskinDepartment of Urology, University of California, San Francisco, San Francisco, CA, USA; Division of Pediatric Urology, University of California San Francisco Benioff Children's Hospital, 550 16th St, 5th Floor, Mission Hall Pediatric Urology, San Francisco, CA 94158, USA. Electronic address: Laurence.baskin@ucsf.edu.

Funding

UCSF KURe Career Development ProgramK12DK083021 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI BASKIN, LAURENCE, CARROLL, PETER R · 2008 to 2017
$5.9M
Hypospadias Differentiation and Endocrine DisruptorsR01DK058105 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI BASKIN, LAURENCE · 2003 to 2015
$2.7M
NIDDK NIH HHS K12 DK083021NIDDK NIH HHS R01 DK058105
6 · The paper itself

Abstract

Recent studies in our lab have utilized three imaging techniques to visualize the developing human fetal urogenital tract in three dimensions: optical projection tomography, scanning electron microscopy and lightsheet fluorescence microscopy. We have applied these technologies to examine changes in morphology and differential gene expression in developing human external genital specimens from the ambisexual stage (<9 weeks fetal age) to well-differentiated male and female organs (>13 weeks fetal age). This work outlines the history and function of each of these three imaging modalities, our methods to prepare specimens for each and the novel findings we have produced thus far. We believe the images in this paper of human fetal urogenital organs produced using lightsheet fluorescence microscopy are the first published to date.

Indexed as

FemaleFetal DevelopmentHumansImaging, Three-DimensionalMaleMicroscopy, Electron, ScanningSex DifferentiationUrogenital SystemHuman fetal urogenita tractLightsheet microscopyOptical projection tomographyScanning electron microscopyThree-dimensional imaging

Identifiers

PMID30262218
PMCPMC6234091

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.