Evidence map›Paper›PMID 30261179›Full record

ArticleMathematical biosciences2018

Phase-plane geometries in coupled enzyme assays.

Justin Eilertsen, Wylie Stroberg, Santiago Schnell

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Mathematical biosciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.7field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 9 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Justin EilertsenDepartment of Molecular & Integrative Physiology, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
Wylie StrobergDepartment of Molecular & Integrative Physiology, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
Santiago SchnellDepartment of Molecular & Integrative Physiology, University of Michigan Medical School, Ann Arbor, MI 48109, USA; Department of Computational Medicine & Bioinformatics, University of Michigan Medical School, Ann Arbor, MI 48109, USA. Electronic address: schnells@umich.edu.
University of Michigan · US

Funding

Michigan IRACDA: Training Future Professors of Engineering and PhysiologyK12GM111725 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI BROOKS, SUSAN V, SEPT, DAVID · 2016 to 2025
$7.9M
NIGMS NIH HHS K12 GM111725
6 · The paper itself

Abstract

The determination of a substrate or enzyme activity by coupling one enzymatic reaction with another easily detectable (indicator) reaction is a common practice in the biochemical sciences. Usually, the kinetics of enzyme reactions is simplified with singular perturbation analysis to derive rate or time course expressions valid under the quasi-steady-state and reactant stationary state assumptions. In this paper, the dynamical behavior of coupled enzyme catalyzed reaction mechanisms is studied by analysis of the phase-plane. We analyze two types of time-dependent slow manifolds - Sisyphus and Laelaps manifolds - that occur in the asymptotically autonomous vector fields that arise from enzyme coupled reactions. Projection onto slow manifolds yields various reduced models, and we present a geometric interpretation of the slow/fast dynamics that occur in the phase-planes of these reactions.

Indexed as

BiocatalysisBiochemical PhenomenaEnzyme ActivationEnzyme AssaysEnzyme PrecursorsEnzymesKineticsMathematical ConceptsModels, BiologicalModels, ChemicalReproducibility of ResultsEnzyme PrecursorsEnzymesAsymptotically autonomous vector fieldCoupled enzyme assaysDifferential-algebraic equationEnzyme kineticsLaelaps manifoldMichaelis–Menten reactionsSisyphus manifoldTime-dependent slow manifold

Identifiers

PMID30261179
PMCPMC6476317
OpenAlexW2792035978

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.