ArticlePloS one2018
Characterization of a leukocidin identified in Staphylococcus pseudintermedius.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 25 citations in OpenAlex.
- Companion animals as potentially important reservoir of methicillin-resistant and virulent strains of coagulase-positive Staphylococcus spp.BMC veterinary research · 2026Article
- Diversity and evolution of leukotoxin operons inmSystems · 2026Article
- Molecular Characterization and Comparative Genomics of TwoVeterinary sciences · 2026Article
- Genomic insights into multidrug resistance and virulence of methicillin-resistantFrontiers in veterinary science · 2026Article
- The dynamic interplay betweenFrontiers in cellular and infection microbiology · 2026Review
- Methicillin Resistance Elements in the Canine PathogenAntibiotics (Basel, Switzerland) · 2024Article
- Detection and characterization of potential virulence determinants inJournal of veterinary science · 2023Article
- Virulence Mechanisms of Staphylococcal Animal Pathogens.International journal of molecular sciences · 2023Review
- Pathogenic potential and antimicrobial resistance of Staphylococcus pseudintermedius isolated from human and animals.Folia microbiologica · 2023Article
- Disturbing the Spatial Organization of Biofilm Communities Affects Expression ofApplied and environmental microbiology · 2023Article
- Antimicrobial activity of α-mangostin againstFrontiers in cellular and infection microbiology · 2023Article
- Article
- Virulence Potential of Biofilm-ProducingAntibiotics (Basel, Switzerland) · 2022Article
- From canines to humans: Clinical importance of Staphylococcus pseudintermedius.PLoS pathogens · 2021Article
- Virulence and Antimicrobial Resistance in CanineMicroorganisms · 2021Article
- Clonal Diversity and Antimicrobial Resistance of Methicillin-ResistantMicroorganisms · 2021Article
- Molecular Epidemiology of Clinical and Colonizing Methicillin-ResistantFrontiers in veterinary science · 2021Article
- Virulence factors in coagulase-positive staphylococci of veterinary interest other thanThe veterinary quarterly · 2020Review
- Population genomics of Staphylococcus pseudintermedius in companion animals in the United States.Communications biology · 2020Article
- Underrated Staphylococcus species and their role in antimicrobial resistance spreading.Genetics and molecular biology · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial infections from Staphylococcus pseudintermedius are the most common cause of skin infections (pyoderma) affecting dogs. Two component pore-forming leukocidins are a family of potent toxins secreted by staphylococci and consist of S (slow) and F (fast) components. They impair the innate immune system, the first line of defense against these pathogens. Seven different leukocidins have been characterized in Staphylococcus aureus, some of which are host and cell specific. Through genome sequencing and analysis of the S. pseudintermedius secretome using liquid chromatography mass spectrometry we identified two proteins, named "LukS-I" and "LukF-I", encoded on a degenerate prophage contained in the genome of S. pseudintermedius isolates. Phylogenetic analysis of LukS-I components in comparison to the rest of the leukocidin family showed that LukS-I was most closely related to S. intermedius LukS-I, S. aureus LukE and LukP, whereas LukF-I was most similar to S. intermedius LukF-I S. aureus gamma hemolysin subunit B. The killing effect of recombinant S. pseudintermedius LukS-I and LukF-I on canine polymorphonuclear leukocytes was determined using a flow cytometry cell permeability assay. The cytotoxic effect occurred only when the two recombinant proteins were combined. Engineered mutant versions of the two-component pore-forming leukocidins, produced through amino acids substitutions at selected points, were not cytotoxic. Anti-Luk-I produced in dogs against attenuated proteins reduced the cytotoxic effect of native canine leukotoxin which highlights the importance of Luk-I as a promising component in a vaccine against canine S. pseudintermedius infections.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.