ArticlePloS one2018
Large scale changes in the transcriptome of Eisenia fetida during regeneration.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 46 citations in OpenAlex.
- Transcriptomic analysis of three annelid species: looking for markers of positional information.BMC genomics · 2026Article
- Integrated transcriptomic and metabolomic ecotoxicity assessment of earthworms exposed to ZnO and Mn-doped ZnO nanomaterials.Frontiers in toxicology · 2026Article
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- A genome resource for the marine annelid Platynereis spp.BMC genomics · 2025Article
- The high-quality telomere-to-telomere genome assembly of the earthworm (Amynthas aspergillum).Scientific data · 2025Article
- SoxC and MmpReg promote blastema formation in whole-body regeneration of fragmenting potworms Enchytraeus japonensis.Nature communications · 2024Article
- Integrative biology of injury in animals.Biological reviews of the Cambridge Philosophical Society · 2023Review
- Wound healing and Cadmium detoxification in the earthwormFrontiers in immunology · 2023Article
- Distinct patterns of gene expression during regeneration and asexual reproduction in the annelid Pristina leidyi.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2022Article
- Importance of clitellar tissue in the regeneration ability of earthworm Eudrilus eugeniae.Functional & integrative genomics · 2022Article
- A pan-metazoan concept for adult stem cells: the wobbling Penrose landscape.Biological reviews of the Cambridge Philosophical Society · 2022Article
- MicroRNAs as Indicators into the Causes and Consequences of Whole-Genome Duplication Events.Molecular biology and evolution · 2022Article
- The Small RNA Universe ofFrontiers in molecular biosciences · 2022Article
- Transcriptional upregulation of multiple earthworm chitinase genes following bacterial challenge suggests their implications in innate immunity.Genes & genomics · 2021Article
- Article
- Comparative Aspects of Annelid Regeneration: Towards Understanding the Mechanisms of Regeneration.Genes · 2021Review
- Animal regeneration in the era of transcriptomics.Cellular and molecular life sciences : CMLS · 2021Review
- Injury-Induced Innate Immune Response During Segment Regeneration of the Earthworm,International journal of molecular sciences · 2021Article
- Panel of serum miRNAs as potential non-invasive biomarkers for pancreatic ductal adenocarcinoma.Scientific reports · 2021Observational
- The transcriptome of anterior regeneration in earthworm Eudrilus eugeniae.Molecular biology reports · 2021Article
Corrections and comments
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Authors and funding
20 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Earthworms show a wide spectrum of regenerative potential with certain species like Eisenia fetida capable of regenerating more than two-thirds of their body while other closely related species, such as Paranais litoralis seem to have lost this ability. Earthworms belong to the phylum Annelida, in which the genomes of the marine oligochaete Capitella telata and the freshwater leech Helobdella robusta have been sequenced and studied. Herein, we report the transcriptomic changes in Eisenia fetida (Indian isolate) during regeneration. Following injury, E. fetida regenerates the posterior segments in a time spanning several weeks. We analyzed gene expression changes both in the newly regenerating cells and in the adjacent tissue, at early (15days post amputation), intermediate (20days post amputation) and late (30 days post amputation) by RNAseq based de novo assembly and comparison of transcriptomes. We also generated a draft genome sequence of this terrestrial red worm using short reads and mate-pair reads. An in-depth analysis of the miRNome of the worm showed that many miRNA gene families have undergone extensive duplications. Sox4, a master regulator of TGF-beta mediated epithelial-mesenchymal transition was induced in the newly regenerated tissue. Genes for several proteins such as sialidases and neurotrophins were identified amongst the differentially expressed transcripts. The regeneration of the ventral nerve cord was also accompanied by the induction of nerve growth factor and neurofilament genes. We identified 315 novel differentially expressed transcripts in the transcriptome, that have no homolog in any other species. Surprisingly, 82% of these novel differentially expressed transcripts showed poor potential for coding proteins, suggesting that novel ncRNAs may play a critical role in regeneration of earthworm.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.