Evidence map›Paper›PMID 30259411›Full record

ArticleMolecular neurobiology2019

Intragenic Transcriptional cis-Antagonism Across SLC6A3.

Ying Zhao, Jinlong Yu, Juan Zhao, Xiaowu Chen, Nian Xiong, Tao Wang, Hong Qing, Zhicheng Lin

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular neurobiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.7field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 7 citations in OpenAlex.

  1. Trial
  2. Article
  3. Article
  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Ying ZhaoLaboratory of Psychiatric Neurogenomics, Basic Neuroscience Division, McLean Hospital, Belmont, MA, 02478, USA.ORCID http://orcid.org/0000-0001-9390-5084
Jinlong YuLaboratory of Psychiatric Neurogenomics, Basic Neuroscience Division, McLean Hospital, Belmont, MA, 02478, USA.
Juan ZhaoLaboratory of Psychiatric Neurogenomics, Basic Neuroscience Division, McLean Hospital, Belmont, MA, 02478, USA.
Xiaowu ChenLaboratory of Psychiatric Neurogenomics, Basic Neuroscience Division, McLean Hospital, Belmont, MA, 02478, USA.
Nian XiongLaboratory of Psychiatric Neurogenomics, Basic Neuroscience Division, McLean Hospital, Belmont, MA, 02478, USA.
Tao WangDepartment of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Hong QingCollege of Life Science, Beijing Institute of Technology, Beijing, 100081, China.
Zhicheng LinLaboratory of Psychiatric Neurogenomics, Basic Neuroscience Division, McLean Hospital, Belmont, MA, 02478, USA. zlin@mclean.harvard.edu.
McLean Hospital · USBeijing Institute of Technology · CNUnion Hospital · CN

Funding

Human dopamine transporter gene: variations and transcriptional regulationR01DA021409 · NIDA · MCLEAN HOSPITAL · PI LIN, ZHICHENG CARL · 2007 to 2024
$4.9M
Monitoring DAT in Live RatsR21DA031573 · NIDA · MCLEAN HOSPITAL · PI LIN, ZHICHENG CARL · 2012 to 2013
$387k
National Institutes of Health DA021409National Institutes of Health DA031573NIDA NIH HHS R01 DA021409NIDA NIH HHS R21 DA031573
6 · The paper itself

Abstract

A promoter can be regulated by various cis-acting elements so that delineation of the regulatory modes among them may help understand developmental, environmental and genetic mechanisms in gene activity. Here we report that the human dopamine transporter gene SLC6A3 carries a 5' distal 5-kb super enhancer (5KSE) which upregulated the promoter by 5-fold. Interestingly, 5KSE is able to prevent 3' downstream variable number tandem repeats (3'VNTRs) from silencing the promoter. This new enhancer consists of a 5'VNTR and three repetitive sub-elements that are conserved in primates. Two of 5KSE's sub-elements, E-9.7 and E-8.7, upregulate the promoter, but only the later could continue doing so in the presence of 3'VNTRs. Finally, E-8.7 is activated by novel dopaminergic transcription factors including SRP54 and Nfe2l1. Together, these results reveal a multimodal regulatory mechanism in SLC6A3.

Indexed as

Promoter Regions, GeneticTranscription, GeneticAdultAnimalsCell Line, TumorDopamine Plasma Membrane Transport ProteinsEnhancer Elements, GeneticHaplotypesHumansMaleMice, Inbred C57BLMinisatellite RepeatsModels, BiologicalTranscription FactorsDopamine Plasma Membrane Transport ProteinsSLC6A3 protein, humanTranscription FactorsEpistasisGenetic markerHaplotypePrimate-specificTransactivation

Identifiers

PMID30259411
PMCPMC6437023
OpenAlexW2893404287

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.