Evidence map›Paper›PMID 30259117›Full record

ArticleMedical molecular morphology2019

Comparing protein and mRNA expressions of the human epidermal growth factor receptor family in estrogen receptor-positive breast cancer.

Sasagu Kurozumi, Yuri Yamaguchi, Hiroshi Matsumoto, Kenichi Inoue, Masafumi Kurosumi, Tetsunari Oyama, Jun Horiguchi, Takaaki Fujii, Ken Shirabe

Abstract readComparative Study
PubMed Publisher
In one paragraph

Article in Medical molecular morphology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Sasagu KurozumiDepartment of General Surgical Science, Gunma University Graduate School of Medicine, 3-39-22, Showa, Maebashi-City, Gunma, 371-8511, Japan. chachagot-a-mail@hotmail.co.jp.ORCID http://orcid.org/0000-0002-9971-2918
Yuri YamaguchiResearch Institute for Clinical Oncology, Saitama Cancer Center, Saitama, Japan.
Hiroshi MatsumotoDivision of Breast Surgery, Saitama Cancer Center, Saitama, Japan.
Kenichi InoueDivision of Breast Oncology, Saitama Cancer Center, Saitama, Japan.
Masafumi KurosumiDepartment of Pathology, Saitama Cancer Center, Saitama, Japan.
Tetsunari OyamaDepartment of Diagnostic Pathology, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan.
Jun HoriguchiDepartment of Breast Surgery, International University of Health and Welfare, Chiba, Japan.
Takaaki FujiiDepartment of General Surgical Science, Gunma University Graduate School of Medicine, 3-39-22, Showa, Maebashi-City, Gunma, 371-8511, Japan.
Ken ShirabeDepartment of General Surgical Science, Gunma University Graduate School of Medicine, 3-39-22, Showa, Maebashi-City, Gunma, 371-8511, Japan.
Gunma University · JPSaitama Cancer Center · JPInternational University of Health and Welfare · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The human epidermal growth factor receptor (HER) family plays a vital role in the development of resistance to treatments in estrogen receptor (ER)-positive breast cancer. This study investigated the correlation between protein and mRNA expressions of the HER family in ER-positive breast cancer. We dissected regions of invasive cancer from the frozen tissues of 34 patients with ER-positive breast cancer using laser-capture microdissection, followed by evaluation of the mRNA levels of the ER and HER family (EGFR, HER2, HER3, and HER4) using the quantitative reverse transcription polymerase chain reaction (qRT-PCR) assay. In addition, we assessed the protein expressions of the ER and HER family using an immunohistochemical (IHC) assay. A significant correlation was observed between the ER protein and mRNA expressions. For HER2, HER3, and HER4, protein expressions significantly correlated with mRNA levels. We established significant correlations of the mRNA level between EGFR versus HER2, as well as EGFR versus HER3. Furthermore, a significant correlation of the mRNA level between HER2 and HER3 was illustrated. In conclusion, IHC evaluation may be reliable and representable for mRNA. Hence, this study established a marked correlation between the mRNA expressions of HER family members in patients with ER-positive breast cancer.

Indexed as

Gene Expression Regulation, NeoplasticAdultAgedAged, 80 and overBreast NeoplasmsErb-b2 Receptor Tyrosine KinasesErbB ReceptorsFemaleHumansImmunohistochemistryMiddle AgedReceptor, ErbB-3Receptor, ErbB-4Receptors, EstrogenRNA, MessengerErb-b2 Receptor Tyrosine KinasesERBB3 protein, humanERBB4 protein, humanErbB ReceptorsReceptor, ErbB-3Receptor, ErbB-4Receptors, EstrogenRNA, MessengerEstrogen receptor-positive breast cancerHuman epidermal growth factor receptorImmunohistochemical assayLaser-capture microdissection methodQuantitative reverse transcription polymerase chain reaction assay

Identifiers

PMID30259117
OpenAlexW2893402394

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.