Evidence map›Paper›PMID 30258682›Full record

ArticleBiomedical optics express2018

Advanced easySTED microscopy based on two-photon excitation by electrical modulations of light pulse wavefronts.

Kohei Otomo, Terumasa Hibi, Yi-Cheng Fang, Jui-Hung Hung, Motosuke Tsutsumi, Ryosuke Kawakami, Hiroyuki Yokoyama, Tomomi Nemoto

Abstract read
In one paragraph

Article in Biomedical optics express, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
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  5. Article
  6. Biophysical research in Okazaki, Japan.Biophysical reviews · 2020
    Review
  7. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Kohei OtomoResearch Institute for Electronic Science, Hokkaido University, Kita 20 Nishi 10, Kita-ku, Sapporo 001-0020, Japan.
Terumasa HibiResearch Institute for Electronic Science, Hokkaido University, Kita 20 Nishi 10, Kita-ku, Sapporo 001-0020, Japan.
Yi-Cheng FangNew Industry Creation Hatchery Center (NICHe), Tohoku University, Aramaki-Aoba 6-6-10, Aoba-ku, Sendai 980-8579, Japan.
Jui-Hung HungNew Industry Creation Hatchery Center (NICHe), Tohoku University, Aramaki-Aoba 6-6-10, Aoba-ku, Sendai 980-8579, Japan.
Motosuke TsutsumiResearch Institute for Electronic Science, Hokkaido University, Kita 20 Nishi 10, Kita-ku, Sapporo 001-0020, Japan.
Ryosuke KawakamiResearch Institute for Electronic Science, Hokkaido University, Kita 20 Nishi 10, Kita-ku, Sapporo 001-0020, Japan.
Hiroyuki YokoyamaNew Industry Creation Hatchery Center (NICHe), Tohoku University, Aramaki-Aoba 6-6-10, Aoba-ku, Sendai 980-8579, Japan.
Tomomi NemotoResearch Institute for Electronic Science, Hokkaido University, Kita 20 Nishi 10, Kita-ku, Sapporo 001-0020, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We developed a compact stimulated emission depletion (STED) two-photon excitation microscopy that utilized electrically controllable components. Transmissive liquid crystal devices inserted directly in front of the objective lens converted the STED light into an optical vortex while leaving the excitation light unaffected. Light pulses of two different colors, 1.06 and 0.64 μm, were generated by laser diode-based light sources, and the delay between the two pulses was flexibly controlled so as to maximize the fluorescence suppression ratio. In our experiments, the spatial resolution of this system was up to three times higher than that obtained without STED light irradiation, and we successfully visualize the fine microtubule network structures in fixed mammalian cells without causing significant photo-damage.

Indexed as

(140.5960) Semiconductor lasers(180.2520) Fluorescence microscopy(180.4315) Nonlinear microscopy(230.3720) Liquid-crystal devices

Identifiers

PMID30258682
PMCPMC6154199

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.