ArticleNature protocols2018
In vivo pulse labeling of isochronic cohorts of cells in the central nervous system using FlashTag.
Article in Nature protocols, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 49 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
49 citing papers in PubMed, 88 citations in OpenAlex.
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- Direct and indirect neurogenesis from radial glial progenitor cell clones in the mouse neocortex.The EMBO journal · 2026Article
- Differentiation timing-dependent axon targeting and subtype specification in retinal ganglion cells.Frontiers in neuroscience · 2026Article
- Integrated ERK-PKA-YAP/TAZ-SHH Signaling Orchestrates Cortical Radial Glia Identity and Lineage Diversification.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Temporal control of progenitor competence shapes maturation in GABAergic neuron development in mice.Nature neuroscience · 2025Article
- TEAD switches interacting partners along neural progenitor lineage progression to execute distinct functions.Genes & development · 2025Article
- Acute dietary methionine restriction triggers cell cycle arrest and reversible growth defects in the neocortex.iScience · 2025Article
- Every cell every gene all at once: Systems genetic approaches toward corticogenesis.Current opinion in neurobiology · 2025Review
- Neural stem cell relay from B1 to B2 cells in the adult mouse ventricular-subventricular zone.Cell reports · 2025Article
- Cell-type-specific roles of FOXP1 in the excitatory neuronal lineage during early neocortical murine development.Cell reports · 2025Article
- Cilia directionality reveals a slow reverse movement of principal neurons for positioning and lamina refinement in the cerebral cortex.Development (Cambridge, England) · 2025Article
- Differential neurogenic patterns underlie the formation of primary and secondary areas in the developing somatosensory cortex.Cerebral cortex (New York, N.Y. : 1991) · 2025Article
- FlashTag-mediated Labeling for Intraventricular Macrophages in the Embryonic Brain.Bio-protocol · 2025Article
- Understanding ubiquitination in neurodevelopment by integrating insights across space and time.Nature structural & molecular biology · 2025Review
- Distinct neurogenic dynamics of cortico-cortical neuronal subtypes in layer 2/3 of the mouse visual cortex.Frontiers in neuroscience · 2025Article
- Lipidomic profiling of mouse brain and human neuron cultures reveals a role forScience translational medicine · 2025Article
- Developmental emergence of first- and higher-order thalamic neuron molecular identities.Development (Cambridge, England) · 2024Article
- Article
- Dysregulation of FLVCR1a-dependent mitochondrial calcium handling in neural progenitors causes congenital hydrocephalus.Cell reports. Medicine · 2024Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The tracing of neuronal cell lineages is critical to our understanding of cellular diversity in the CNS. This protocol describes a fluorescence birth-dating technique to label, track and isolate isochronic cohorts of newborn cells in the CNS in vivo in mouse embryos. Injection of carboxyfluorescein esters (CFSEs) into the cerebral ventricle allows pulse labeling of mitotic (M phase) ventricular zone (VZ) progenitors and their progeny across the CNS, a procedure we termed FlashTag. Specificity for M-phase apical progenitors is a result of the somata of these cells transiently contacting the ventricular wall during this cell-cycle phase, exposing them to CFSE injected into the cerebrospinal fluid. Using this approach, the developmental trajectory of progenitors and their daughter neurons can be tracked. Labeled cells can be imaged ex vivo or in fixed tissue, targeted for electrophysiological experiments or isolated using FACS for cell culture or (single-cell) RNA sequencing. Multiple embryos can be labeled within 30 min. The dye is retained for several weeks, allowing labeled cells to be identified postnatally. This protocol describes the labeling procedure using in utero injection, the isolation of live cells using FACS and the processing of labeled tissue for immunohistochemistry.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.