ArticleScientific reports2018
Biolistic-delivery-based transient CRISPR/Cas9 expression enables in planta genome editing in wheat.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
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Who cites it
71 citing papers in PubMed.
- Wheat's Up with CRISPR-Cas-Current Advances, Obstacles and Perspectives.International journal of molecular sciences · 2026Review
- Recent advances in generation of doubled haploid plants for genetic improvement in solanaceous vegetable crops.Planta · 2026Review
- From recalcitrance to precision: a robust regeneration, transformation and targeted gene editing framework inFrontiers in genome editing · 2026Article
- Development ofFrontiers in genome editing · 2026Article
- Advances in the use of morphogenic regulators and peptide regenerating factors for boosting plant transformation and genome editing.Frontiers in plant science · 2026Review
- Beyond GMOs: transgene-free gene-edited crops for global food security.Frontiers in plant science · 2026Review
- CRISPR-Cas technologies for precision genome editing in plants: advances, applications, and future perspectives.Frontiers in plant science · 2026Review
- CRISPR Technology: Transforming the Future of Medicine and Diagnostics.Biochemistry · 2025Review
- Enhancing biolistic plant transformation and genome editing with a flow guiding barrel.Nature communications · 2025Article
- CRISPR-Cas applications in agriculture and plant research.Nature reviews. Molecular cell biology · 2025Review
- Hazelnut allergome overview and Cor a gRNAs identification.BMC plant biology · 2025Article
- An efficient mRNA delivery system for genome editing in plants.Plant biotechnology journal · 2025Article
- Current Advancement and Future Prospects in Simplified Transformation-Based Plant Genome Editing.Plants (Basel, Switzerland) · 2025Review
- Review
- Genome editing in cells of apple cultivar 'Fuji' using geminivirus-derived replicons for transient expression of CRISPR/Cas9 components.Plant biotechnology (Tokyo, Japan) · 2024Article
- Direct Nuclear Delivery of Proteins on Living Plant via Partial Enzymatic Cell Wall Digestion.Current issues in molecular biology · 2024Article
- Plant regeneration in the new era: from molecular mechanisms to biotechnology applications.Science China. Life sciences · 2024Review
- A comprehensive review of in planta stable transformation strategies.Plant methods · 2024Review
- Creating Climate-Resilient Crops by Increasing Drought, Heat, and Salt Tolerance.Plants (Basel, Switzerland) · 2024Review
- Article
11 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The current application of genome editing to crop plants is limited to cultivars that are amenable to in vitro culture and regeneration. Here, we report an in planta genome-editing which does not require callus culture and regeneration. Shoot apical meristems (SAMs) contain a subepidermal cell layer, L2, from which germ cells later develop during floral organogenesis. The biolistic delivery of gold particles coated with plasmids expressing CRISPR/Cas9 components designed to target TaGASR7 were bombarded into SAM-exposed embryos of imbibed seeds. Bombarded embryos showing transient GFP expression within SAM were selected and grown into adult plants. Mutations in the target gene were assessed in fifth-leaf tissue by cleaved amplified polymorphic sequence analysis. Eleven (5.2%) of the 210 bombarded plants carried mutant alleles, and the mutations of three (1.4%) of these were inherited in the next generation. Genotype analysis of T
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