ArticleScientific reports2018
Fecal source tracking and eDNA profiling in an urban creek following an extreme rain event.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Environmental DNA (eDNA) from humans: Review of recent advancements and forensic implications.International journal of legal medicine · 2026Review
- Impact of Rainfall on Aquatic and Terrestrial Species Monitoring Using Riverine Environmental DNA.Ecology and evolution · 2026Article
- Molecular exploration of mosquitoes and their vector-borne pathogens in urban water environments in Benin.Scientific reports · 2026Article
- Optimization of a Semi-nested PCR Protocol for Amplifying the Entire Spike Protein Region to Identify SARS-CoV-2 Variants in Wastewater.Food and environmental virology · 2026Article
- Elusive tropical forest canopy diversity revealed through environmental DNA contained in rainwater.Science advances · 2025Article
- Article
- Spatial and Temporal Dynamics of Chemical and Microbial Contamination in Nonurban Floodwaters.Environmental science & technology · 2024Article
- A comparative study on eDNA-based detection of Siamese bat catfish (Oreoglanis siamensis) in wet and dry conditions.Scientific reports · 2024Article
- Identification of potential microbial risk factors associated with fecal indicator exceedances at recreational beaches.Environmental microbiome · 2024Article
- eDNA profiling of mammals, birds, and fish of surface waters by mitochondrial metagenomics: application for source tracking of fecal contamination in surface waters.Environmental monitoring and assessment · 2022Article
- Variable fecal source prioritization in recreational waters routinely monitored with viral and bacterial general indicators.Water research · 2021Article
- Microbial source tracking using metagenomics and other new technologies.Journal of microbiology (Seoul, Korea) · 2021Review
- Human Fecal Pollution Monitoring and Microbial Risk Assessment for Water Reuse Potential in a Coastal Industrial-Residential Mixed-Use Watershed.Frontiers in microbiology · 2021Article
- Predicting the fate of eDNA in the environment and implications for studying biodiversity.Proceedings. Biological sciences · 2019Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fecal contamination of recreational waters (i.e. lakes, rivers, beaches) poses an on-going problem for environmental and public health. Heavy rainfall can exacerbate existing problems with fecal contamination. As there could be variable sources of fecal contamination, identifying the source is critical for remediation efforts. This study utilized microbial source tracking (MST), chemical source tracking (CST) markers and environmental DNA (eDNA) metabarcoding to profile sampling areas and identify sources of fecal contamination in creek, stormwater outfall and beach sites in the Etobicoke Creek watershed (Toronto, ON). Water samples were collected before and immediately following an extreme rain event. MST and CST identified stormwater outfalls as an important source of human fecal contamination during dry and wet conditions. eDNA metabarcoding allowed for potential identification of additional sources of fecal contamination and provided additional evidence of human fecal contamination. The extreme rainfall event altered the eDNA profiles, causing creek and beach sites to reflect a greater diversity of mammal and bird eDNA sequences. The profiles provided by eDNA metabarcoding provide a proof of concept suggesting that eDNA metabarcoding can be a useful tool to complement MST and CST methods for profiling sources of fecal contamination and studying impacts of extreme rain events.
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