ArticleStem cell research & therapy2018
Organ culture of seminiferous tubules using a modified soft agar culture system.
Article in Stem cell research & therapy, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 36 citations in OpenAlex.
- Use of organotypic or three-dimensional systems with basic fibroblast growth factor for in vitro culture of immature collared peccary testicle.Reproduction & fertility · 2025Article
- Infertility treatment using polysaccharides-based hydrogels: new strategies in tissue engineering and regenerative medicine.Journal of nanobiotechnology · 2025Review
- Recent Progress of Induced Spermatogenesis In Vitro.International journal of molecular sciences · 2024Review
- Advances of three-dimensional (3D) culture systems for in vitro spermatogenesis.Stem cell research & therapy · 2023Review
- Culture-space control is effective in promoting haploid cell formation and spermiogenesis in vitro in neonatal mice.Scientific reports · 2023Article
- A pumpless monolayer microfluidic device based on mesenchymal stem cell-conditioned medium promotes neonatal mouse in vitro spermatogenesis.Stem cell research & therapy · 2023Article
- Biomaterials for Testicular Bioengineering: How far have we come and where do we have to go?Frontiers in endocrinology · 2023Review
- Article
- Mesenchymal stem cells promote spermatogonial stem/progenitor cell pool and spermatogenesis in neonatal mice in vitro.Scientific reports · 2022Article
- Effect of NMDA receptor agonist and antagonist on spermatogonial stem cells proliferation in 2- and 3- dimensional culture systems.Molecular biology reports · 2022Article
- Strategies for cryopreservation of testicular cells and tissues in cancer and genetic diseases.Cell and tissue research · 2021Review
- Review
- Engineered reproductive tissues.Nature biomedical engineering · 2020Review
- Comparison of PLZF Gene Expression between Pluripotent Stem Cells and Testicular Germ Cells.Cell journal · 2020Article
- The air-liquid interface culture of the mechanically isolated seminiferous tubules embedded in agarose or alginate improves in vitro spermatogenesis at the expense of attenuating their integrity.In vitro cellular & developmental biology. Animal · 2020Article
- Three-dimensional testicular organoids as novelEnvironmental epigenetics · 2019Review
- In Vitro Spermatogenesis by Three-dimensional Culture of Spermatogonial Stem Cells on Decellularized Testicular Matrix.Galen medical journal · 2019Article
- Advanced bioengineering of male germ stem cells to preserve fertility.Journal of tissue engineeringReview
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn-vitro spermatogenesis in mammalian species is considered an important topic in reproductive biology. New strategies for achieving a complete version of spermatogenesis ex vivo have been conducted using an organ culture method or culture of testicular cells in a three-dimensional soft agar culture system (SACS). The aim of this study was to develop a new method that supports spermatogenesis to the meiotic phase and morphologically mature spermatozoa through the culture of testicular cells and seminiferous tubules (STs) in a modified SACS, respectively.
methodsFirst, enzymatically dissociated testicular cells and mechanically dissociated STs of neonatal mice were separately embedded in agarose and then placed on the flat surface of agarose gel half-soaked in the medium to continue culture with a gas-liquid interphase method.
resultsFollowing 40 days of culture, the meiotic (Scp3) and post-meiotic (Acr) gene expression in aggregates and STs was confirmed by real-time polymerase chain reaction. These results were complemented by immunohistochemistry. The presence of morphologically mature spermatozoa in the frozen sections of STs was demonstrated with hematoxylin and eosin staining. We observed Plzf- or Integrin α6-positive spermatogonia in both cultures after 40 days, indicating the potency of the culture system for both self-renewal and differentiation.
conclusionsThis technique can be used as a valuable approach for performing research on spermatogenesis and translating it into the human clinical setting.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.