Evidence map›Paper›PMID 30257710›Full record

ArticleParasites & vectors2018

Erythrocyte-binding assays reveal higher binding of Plasmodium knowlesi Duffy binding protein to human Fy

Mun Yik Fong, Fei Wen Cheong, Yee Ling Lau

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Parasites & vectors, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.7field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Two Genetically DistinctThe American journal of tropical medicine and hygiene · 2020
    Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Mun Yik FongDepartment of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. fongmy@um.edu.my.ORCID http://orcid.org/0000-0001-5934-2724
Fei Wen Cheong
Yee Ling LauDepartment of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
University of Malaya · MY

Funding

Fundamental Research Grant Scheme, Ministry of Higher Education, Malaysia FP036-2015AUniversity Malaya Frontier Research Grant 2017 FG015-17AFR
6 · The paper itself

Abstract

backgroundThe merozoite of the zoonotic Plasmodium knowlesi invades human erythrocytes via the binding of its Duffy binding protein (PkDBPαII) to the Duffy antigen on the eythrocytes. The Duffy antigen has two immunologically distinct forms, Fy

resultsIn the erythrocyte-binding assay, binding level was determined by scoring the number of rosettes that were formed by erythrocytes surrounding transfected mammalian COS-7 cells which expressed PkDBPαII. The assay result revealed a significant difference in the binding level. The number of rosettes scored for Fy

conclusionsThe erythrocyte-binding assay provided a simple approach to quantitatively determine the binding level of PkDBPαII to the erythrocyte Duffy antigen. Using this assay, PkDBPαII was found to display higher binding to Fy

Indexed as

AllelesAnimalsAntigens, ProtozoanChlorocebus aethiopsCOS CellsDuffy Blood-Group SystemErythrocytesHumansPhenotypePlasmodium knowlesiProtein BindingProtozoan ProteinsReceptors, Cell SurfaceAntigens, ProtozoanDuffy antigen binding protein, PlasmodiumDuffy Blood-Group SystemProtozoan ProteinsReceptors, Cell SurfaceDuffy antigenDuffy binding proteinErythrocyte-binding assayPlasmodium knowlesi

Identifiers

PMID30257710
PMCPMC6158824
OpenAlexW2893572632

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.