ArticleNucleic acids research2019
Targeting individual cells by barcode in pooled sequence libraries.
Article in Nucleic acids research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed.
- Targeted single-cell RNA sequencing of transcription factors enhances the identification of cell types and trajectories.Genome research · 2021Article
- Modular barcode beads for microfluidic single cell genomics.Scientific reports · 2021Article
- Article
- Single-cell analysis of a mutant library generated using CRISPR-guided deaminase in human melanoma cells.Communications biology · 2020Article
- Article
- Recovery and analysis of transcriptome subsets from pooled single-cell RNA-seq libraries.Nucleic acids research · 2019Article
- Multiplexed enrichment and genomic profiling of peripheral blood cells reveal subset-specific immune signatures.Science advances · 2019Article
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Authors and funding
4 authors.
Funding
Abstract
Transcriptional profiling of thousands of single cells in parallel by RNA-seq is now routine. However, due to reliance on pooled library preparation, targeting analysis to particular cells of interest is difficult. Here, we present a multiplexed PCR method for targeted sequencing of select cells from pooled single-cell sequence libraries. We demonstrated this molecular enrichment method on multiple cell types within pooled single-cell RNA-seq libraries produced from primary human blood cells. We show how molecular enrichment can be combined with FACS to efficiently target ultra-rare cell types, such as the recently identified AXL+SIGLEC6+ dendritic cell (AS DC) subset, in order to reduce the required sequencing effort to profile single cells by 100-fold. Our results demonstrate that DNA barcodes identifying cells within pooled sequencing libraries can be used as targets to enrich for specific molecules of interest, for example reads from a set of target cells.
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