ArticlePloS one2018
Characterization of novel glycosyl hydrolases discovered by cell wall glycan directed monoclonal antibody screening and metagenome analysis of maize aerial root mucilage.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Nitrogen fixation rates and aerial root production among maize landraces.Frontiers in plant science · 2025Article
- Polysaccharide utilization loci encoded DUF1735 likely functions as membrane-bound spacer for carbohydrate active enzymes.FEBS open bio · 2024Article
- Identification of genetic and environmental factors influencing aerial root traits that support biological nitrogen fixation in sorghum.G3 (Bethesda, Md.) · 2024Article
- Probiotic model for studying rhizosphere interactions of root exudates and the functional microbiome.The ISME journal · 2024Article
- Growth rate is a dominant factor predicting the rhizosphere effect.The ISME journal · 2023Article
- Microbiota-mediated nitrogen fixation and microhabitat homeostasis in aerial root-mucilage.Microbiome · 2023Article
- Structure and function of microbial α-l-fucosidases: a mini review.Essays in biochemistry · 2023Review
- Understanding the structure and composition of recalcitrant oligosaccharides in hydrolysate using high-throughput biotin-based glycome profiling and mass spectrometry.Scientific reports · 2022Article
- Recent Advances in Agronomic and Physio-Molecular Approaches for Improving Nitrogen Use Efficiency in Crop Plants.Frontiers in plant science · 2022Review
- Characterization of a highly xylose tolerant β-xylosidase isolated from high temperature horse manure compost.BMC biotechnology · 2021Article
- Biochemical and Genetic Approaches Improving Nitrogen Use Efficiency in Cereal Crops: A Review.Frontiers in plant science · 2021Review
- Meta-QTL Analysis in Rice and Cross-Genome Talk of the Genomic Regions Controlling Nitrogen Use Efficiency in Cereal Crops Revealing Phylogenetic Relationship.Frontiers in genetics · 2021Article
- Biogels in Soils: Plant Mucilage as a Biofilm Matrix That Shapes the Rhizosphere Microbial Habitat.Frontiers in plant science · 2021Article
- Identification of Nitrogen Fixation Genes inMicroorganisms · 2020Article
- Complete Genome Sequence ofMicrobiology resource announcements · 2020Article
- Genomic characterization of a diazotrophic microbiota associated with maize aerial root mucilage.PloS one · 2020Article
- Mucilage Polysaccharide Composition and Exudation in Maize From Contrasting Climatic Regions.Frontiers in plant science · 2020Article
- Identification of quantitative trait loci associated with nitrogen use efficiency in winter wheat.PloS one · 2020Article
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5 authors.
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Abstract
An indigenous maize landrace from the Sierra Mixe region of Oaxaca, Mexico exhibits extensive formation of aerial roots which exude large volumes of a polysaccharide-rich gel matrix or "mucilage" that harbors diazotrophic microbiota. We hypothesize that the mucilage associated microbial community carries out multiple functions, including disassembly of the mucilage polysaccharide. In situ, hydrolytic assay of the mucilage revealed endogenous arabinofuranosidase, galactosidase, fucosidase, mannosidase and xylanase activities. Screening the mucilage against plant cell wall glycan-specific monoclonal antibodies recognized the presence of carbohydrate epitopes of hemicellulosic polysaccharides like xyloglucan (both non-fucosylated and fucosylated), xylan (both substituted and unsubstituted xylan domains) and pectic-arabinogalactans, all of which are potential carbon sources for mucilage microbial residents. Mucilage metagenome annotation using MG-RAST identified the members forming the microbial community, and gene fragments with predicted functions associated with carbohydrate disassembly. Data from the in situ hydrolytic activity and monoclonal antibody screening assays were used to guide the selection of five full length genes with predicted glycosyl hydrolase function from the GenBank database that were similar to gene fragments of high relative abundance in the mucilage metagenomes. These five genes were then synthesized for recombinant production in Escherichia coli. Here we report the characterization of an α-N-arabinofuranosidase (GH51) and an oligosaccharide reducing-end xylanase (GH8) from Flavobacterium johnsoniae; an α-L-fucosidase (GH29) and a xylan β-1,4 xylosidase (GH39) from Spirosoma linguale, and a β-mannosidase (GH2) from Agrobacterium fabrum. Biochemical characterization of these enzymes revealed a β-Mannosidase that also exhibits a secondary activity towards the cleavage of galactosyl residues. We also describe two xylanases (GH8 and GH39) from underexplored glycosyl hydrolase families, one thermostable α-L-Fucosidase (GH29) and a thermostable α-N-Arabinofuranosidase (GH51).
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