Evidence map›Paper›PMID 30254741›Full record

ArticleF1000Research2018

FastQ Screen: A tool for multi-genome mapping and quality control.

Steven W Wingett, Simon Andrews

Abstract read
In one paragraph

Article in F1000Research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1,352 papers.

0numbers the graph read from it
0cells of the map it votes in
1,352citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1,352 citing papers in PubMed.

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  10. Analysis of bacteriophage diversity in Ganga river ecosystem: insights from metagenome-based sequencing.International microbiology : the official journal of the Spanish Society for Microbiology · 2026
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  16. Spindle Cell Tumors with aInternational journal of molecular sciences · 2026
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  18. Draft genome sequences of eight biofilm-formingMicrobiology resource announcements · 2026
    Article
  19. Article
  20. Article

1,292 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Steven W WingettBioinformatics, Babraham Institute, Cambridge, CB22 3AT, UK.ORCID 0000-0002-2343-0711
Simon AndrewsBioinformatics, Babraham Institute, Cambridge, CB22 3AT, UK.

Funding

Biotechnology and Biological Sciences Research Council BBS/E/B/000C05Medical Research Council G0801156
6 · The paper itself

Abstract

DNA sequencing analysis typically involves mapping reads to just one reference genome. Mapping against multiple genomes is necessary, however, when the genome of origin requires confirmation. Mapping against multiple genomes is also advisable for detecting contamination or for identifying sample swaps which, if left undetected, may lead to incorrect experimental conclusions. Consequently, we present FastQ Screen, a tool to validate the origin of DNA samples by quantifying the proportion of reads that map to a panel of reference genomes. FastQ Screen is intended to be used routinely as a quality control measure and for analysing samples in which the origin of the DNA is uncertain or has multiple sources.

Indexed as

High-Throughput Nucleotide SequencingSoftwareChromosome MappingGenomeQuality ControlSequence Analysis, DNABioinformatics Contamination FastQC Illumina Metagenomics NGS QC Sequencing

Identifiers

PMID30254741
PMCPMC6124377

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.