ArticleInternational journal of nanomedicine2018
Establishment of a novel homogeneous nanoparticle-based assay for sensitive procalcitonin detection of ultra low-volume serum samples.
Article in International journal of nanomedicine, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 31 citations in OpenAlex.
- Recent advances in the biosensing platforms for sepsis diagnosis.RSC advances · 2026Review
- High procalcitonin level is related to blood stream infections, gram-negative pathogens, and ICU admission in infections of adult febrile cancer patients.Journal of the Egyptian National Cancer Institute · 2025Article
- Development and application of an amplified luminescent proximity homogeneous assay-linked immunosorbent assay for the accurate quantification of kidney injury molecule-1.Frontiers in molecular biosciences · 2023Article
- Development of a rapid homogeneous immunoassay for detection of rotavirus in stool samples.Frontiers in public health · 2022Article
- Development of an amplified luminescent proximity homogeneous assay for the detection of sulfonamides in animal-derived products.Food science & nutrition · 2021Article
- Sepsis diagnosis and treatment using nanomaterials.Biomedical engineering letters · 2021Review
- MicroRNA-155: Regulation of Immune Cells in Sepsis.Mediators of inflammation · 2021Review
- Spiegelmer-Based Sandwich Assay for Cardiac Troponin I Detection.International journal of molecular sciences · 2020Article
- Development of a Novel Homogeneous Nanoparticle-Based Assay for Rapid and High-Throughput Quantitation of the sST2 Protein in Human Serum.International journal of nanomedicine · 2020Article
- Comparison of Bead-Based Fluorescence Versus Planar Electrochemiluminescence Multiplex Immunoassays for Measuring Cytokines in Human Plasma.Frontiers in immunology · 2020Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeSepsis is a potentially fatal systemic body infection with a significant mortality rate worldwide. Although C-reactive protein (CRP), interleukin-6 (IL-6), and procalcitonin (PCT) might be biomarkers for sepsis diagnosis, PCT is more sensitive and specific than CRP or IL-6. We aimed to establish an efficient immunoassay that precisely detects PCT in human serum for the early diagnosis of sepsis. MATERIALS AND
methodsWe developed a novel amplified luminescent proximity homogeneous assay (AlphaLISA) for the quantitative detection of PCT in serum. In this assay, a pair of antibodies was used to capture PCT in serum and to form sandwich complexes after incubating for 15 minutes at 37°C.
resultsPCT concentrations were determined within a linear range of 0.016-100 ng/mL. The limit of detection was 18.6 pg/mL. The results demonstrate that the reproducibility, recovery, and specificity of this assay for PCT meet the requirements of clinical detection. The coefficient of determination (
conclusionThe novel assay for PCT detection was robust with high sensitivity and a broad dynamic range. Compared with conventional heterogeneous detection methods such as ELISA, this assay measured the concentration of the homogeneous form of PCT and provided results that are more accurate within a shorter detection time. We expect that this novel method will be useful for the early screening and prognosis evaluation of patients with sepsis.
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