ArticleBMC biotechnology2018
CRISPR/Cas9-mediated genome editing induces exon skipping by complete or stochastic altering splicing in the migratory locust.
Article in BMC biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 38 citations in OpenAlex.
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- Escaping from CRISPR-Cas-mediated knockout: the facts, mechanisms, and applications.Cellular & molecular biology letters · 2024Review
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- Effect of Lacking ZKSCAN3 on Autophagy, Lysosomal Biogenesis and Senescence.International journal of molecular sciences · 2023Article
- Knock down of TIMP-2 by siRNA and CRISPR/Cas9 mediates diverse cellular reprogramming of metastasis and chemosensitivity in ovarian cancer.Cancer cell international · 2022Article
- Recruitment of a splicing factor to the nuclear lamina for its inactivation.Communications biology · 2022Article
- Functional Divergence of the bag-of-marbles Gene in the Drosophila melanogaster Species Group.Molecular biology and evolution · 2022Article
- In rice splice variants that restore the reading frame after frameshifting indel introduction are common, often induced by the indels and sometimes lead to organism-level rescue.PLoS genetics · 2022Article
- Chemical Ecology and Olfaction in Short-Horned Grasshoppers (Orthoptera: Acrididae).Journal of chemical ecology · 2022Review
- Non-genetic and genetic rewiring underlie adaptation to hypomorphic alleles of an essential gene.The EMBO journal · 2021Article
- Highly efficient manipulation of nervous system gene expression with NEPTUNE.Cell reports methods · 2021Article
- A novel tissue specific alternative splicing variant mitigates phenotypes in Ets2 frame-shift mutant models.Scientific reports · 2021Article
- Machine learning based CRISPR gRNA design for therapeutic exon skipping.PLoS computational biology · 2021Article
- Discovering and validating cancer genetic dependencies: approaches and pitfalls.Nature reviews. Genetics · 2020Review
- Developing oncolyticIranian journal of basic medical sciences · 2020Article
- Development of CRISPR/Cas9 system for targeted DNA modifications and recent improvements in modification efficiency and specificity.BMB reports · 2020Review
- Applying switchable Cas9 variants to in vivo gene editing for therapeutic applications.Cell biology and toxicology · 2020Review
- Revisiting CRISPR/Cas-mediated crop improvement: Special focus on nutrition.Journal of biosciences · 2020Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe CRISPR/Cas9 system has been widely used to generate gene knockout/knockin models by inducing frameshift mutants in cell lines and organisms. Several recent studies have reported that such mutants can lead to in-frame exon skipping in cell lines. However, there was little research about post-transcriptional effect of CRISPR-mediated gene editing in vivo.
resultsWe showed that frameshift indels also induced complete or stochastic exon skipping by deleting different regions to influence pre-mRNA splicing in vivo. In the migratory locust, the missing 55 bp at the boundary of intron 3 and exon 4 of an olfactory receptor gene, LmigOr35, resulted in complete exon 4 skipping, whereas the lacking 22 bp in exon 4 of LmigOr35 only resulted in stochastic exon 4 skipping. A single sgRNA induced small insertions or deletions at the boundary of intron and exon to disrupt the 3' splicing site causing completely exon skipping, or alternatively induce small insertions or deletions in the exon to stochastic alter splicing causing the stochastic exon skipping.
conclusionsThese results indicated that complete or stochastic exon skipping could result from the CRISPR-mediated genome editing by deleting different regions of the gene. Although exon skipping caused by CRISPR-mediated editing was an unexpected outcome, this finding could be developed as a technology to investigate pre-mRNA splicing or to cure several human diseases caused by splicing mutations.
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