ArticleJournal of the American Chemical Society2018
Outer Membrane Translocon Communicates with Inner Membrane ATPase To Stop Lipopolysaccharide Transport.
Article in Journal of the American Chemical Society, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 29 citations in OpenAlex.
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- Fluconazole-Decorated Gold Nanoparticles as a Novel Therapeutic Strategy Against Carbapenem-ResistantInfection and drug resistance · 2026Article
- Engineering a Gram-Negative Bactericidal Hydrogel: CuResearch (Washington, D.C.) · 2026Article
- Structural snapshots of Pseudomonas aeruginosa LptBNature communications · 2025Article
- Article
- Inhibiting Lipopolysaccharide Biogenesis: The More You Know the Further You Go.Annual review of biochemistry · 2025Review
- Determination of Initial Rates of Lipopolysaccharide Transport.Biochemistry · 2024Article
- Article
- ABC Transporters in Bacterial Nanomachineries.International journal of molecular sciences · 2023Review
- Antibiofilm property and multiple action of peptide PEW300 againstFrontiers in microbiology · 2022Article
- Methods for the solubilisation of membrane proteins: the micelle-aneous world of membrane protein solubilisation.Biochemical Society transactions · 2021Review
- Assembly and Maintenance of Lipids at the Bacterial Outer Membrane.Chemical reviews · 2021Review
- Article
- Transport of lipopolysaccharides and phospholipids to the outer membrane.Current opinion in microbiology · 2021Review
- Dynamics of an LPS translocon induced by substrate and an antimicrobial peptide.Nature chemical biology · 2021Article
- Degradation of Components of the Lpt Transenvelope Machinery Reveals LPS-Dependent Lpt Complex Stability inFrontiers in molecular biosciences · 2021Article
- Phospholipid transporter shifts into reverse.Nature structural & molecular biology · 2021Article
- How the assembly and protection of the bacterial cell envelope depend on cysteine residues.The Journal of biological chemistry · 2020Review
- Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The survival of Gram-negative bacteria depends on assembly of the asymmetric outer membrane, which creates a barrier that prevents entry of toxic molecules including antibiotics. The outer leaflet of the outer membrane is composed of lipopolysaccharide, which is made at the inner membrane and pushed across a protein bridge that spans the inner and outer membranes. We have developed a fluorescent assay to follow lipopolysaccharide (LPS) transport across a bridge linking proteoliposomes that mimic the inner and outer membranes. We show that LPS is delivered to the leaflet of the outer membrane proteoliposome that corresponds to the outer leaflet of the membrane in a cell. Transport stops long before substrates at the inner membrane are exhausted. Using mutants of the transport machinery, we find that the final amount of LPS delivered into the membrane depends on the affinity of the outer membrane translocon for LPS. Furthermore, ATP hydrolysis depends on delivery of LPS into the outer membrane. Therefore, the transport process is regulated by the outer membrane translocon causing ATP hydrolysis in the inner membrane proteoliposome to stop. Negative feedback from the outer membrane to the inner membrane provides a mechanism for long distance control over LPS transport.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.