ArticleNucleic acids research2018
Monitored eCLIP: high accuracy mapping of RNA-protein interactions.
Article in Nucleic acids research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed.
- Assembly of exon junction complexes on spliceosomes begins before spliceosome activation.Nucleic acids research · 2026Article
- Signal or noise? RNA-binding proteins and the challenges of binding site assignments.Nucleic acids research · 2026Review
- Review
- Gene regulation through exon junction complex modularity.Nature structural & molecular biology · 2025Review
- Chemical inhibition of exon junction complex assembly impairs mRNA localization and neural stem cells ciliogenesis.Nucleic acids research · 2025Article
- Exon-junction complex association with stalled ribosomes and slow translation-independent disassembly.Nature communications · 2024Article
- MRM-BERT: a novel deep neural network predictor of multiple RNA modifications by fusing BERT representation and sequence features.RNA biology · 2024Article
- Comprehensive mapping of exon junction complex binding sites reveals universal EJC deposition in Drosophila.BMC biology · 2023Article
- An RNA tagging approach for system-wide RNA-binding proteome profiling and dynamics investigation upon transcription inhibition.Nucleic acids research · 2021Article
- NCBP3 positively impacts mRNA biogenesis.Nucleic acids research · 2020Article
- Structural and functional insights into CWC27/CWC22 heterodimer linking the exon junction complex to spliceosomes.Nucleic acids research · 2020Article
- Understanding Long Noncoding RNA and Chromatin Interactions: What We Know So Far.Non-coding RNA · 2019Review
- Methods to study RNA-protein interactions.Nature methods · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CLIP-seq methods provide transcriptome-wide snapshots of RNA-protein interactions in live cells. Reverse transcriptases stopping at cross-linked nucleotides sign for RNA-protein binding sites. Reading through cross-linked positions results in false binding site assignments. In the 'monitored enhanced CLIP' (meCLIP) method, a barcoded biotinylated linker is ligated at the 5' end of cross-linked RNA fragments to purify RNA prior to the reverse transcription. cDNAs keeping the barcode sequence correspond to reverse transcription read-throughs. Read through occurs in unpredictable proportions, representing up to one fourth of total reads. Filtering out those reads strongly improves reliability and precision in protein binding site assignment.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.