ReviewFrontiers in microbiology2018
Recent Advances on the Multiplex Molecular Detection of Plant Viruses and Viroids.
Review in Frontiers in microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
41 citing papers in PubMed, 1 synthesis or guideline pooled it, 112 citations in OpenAlex.
- Pooled it
- Detection Strategies for Begomoviruses: A Focus Review of Methods.Molecular biotechnology · 2026Review
- Two-step Reverse Transcription-multiplex Polymerase Chain Reaction Assay for the Simultaneous Detection of Seven Grapevine-infecting Viruses.Current microbiology · 2026Article
- Comprehensive virome analysis of potato crops in Bangladesh reveals diverse viral communities and the first report of potato virus V.Virology journal · 2026Article
- Investigating the Occurrence of Viruses in Sweet Cherry in China and Developing Multiplex RT-PCR Assays for Their Detection.Plants (Basel, Switzerland) · 2025Article
- Molecular characterization and multiplex PCR assay for the detection and identification of three different begomovirus species in the chilli fields of Tamil Nadu.Molecular biology reports · 2025Article
- Viral Challenges in Wheat: Comprehensive Diagnosis and Innovative Management Approaches.Current microbiology · 2025Review
- Article
- Article
- Viroscope™: a universal solution for plant virus and viroid diagnostics using HTS and cloud-based analysis.Frontiers in microbiology · 2025Article
- Development of a TaqMan qPCR for the Simultaneous Detection of the TuMV and BBWV2 Viruses Responsible for the Viral Disease inMicroorganisms · 2024Article
- Article
- An evaluation of nucleic acid-based molecular methods for the detection of plant viruses: a systematic review.Virusdisease · 2024Review
- Occurrence and Multiplex PCR Detection of Citrus Yellow Vein Clearing Virus in Korea.The plant pathology journal · 2024Article
- Article
- Whole genome characterization and diagnostics of prunus necrotic ringspot virus (PNRSV) infecting apricot in India.Scientific reports · 2023Article
- Raman-dielectrophoresis goes viral: towards a rapid and label-free platform for plant virus characterization.Frontiers in microbiology · 2023Article
- A Simplified Multiplex PCR Assay for Simultaneous Detection of Six Viruses Infecting Diverse Chilli Species in India and Its Application in Field Diagnosis.Pathogens (Basel, Switzerland) · 2022Article
- Development of a multiplex RT-PCR assay for simultaneous detection of Lily symptomless virus, Lily mottle virus, Cucumber mosaic virus, and Plantago asiatica mosaic virus in Lilies.Virology journal · 2022Article
- Real-Time On-Site Diagnosis of Quarantine Pathogens in Plant Tissues by Nanopore-Based Sequencing.Pathogens (Basel, Switzerland) · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant viruses are still one of the main contributors to economic losses in agriculture. It has been estimated that plant viruses can cause as much as 50 billion euros loss worldwide, per year. This situation may be worsened by recent climate change events and the associated changes in disease epidemiology. Reliable and early detection methods are still one of the main and most effective actions to develop control strategies for plant viral diseases. During the last years, considerable progress has been made to develop tools with high specificity and low detection limits for use in the detection of these plant pathogens. Time and cost reductions have been some of the main objectives pursued during the last few years as these increase their feasibility for routine use. Among other strategies, these objectives can be achieved by the simultaneous detection and (or) identification of several viruses in a single assay. Nucleic acid-based detection techniques are especially suitable for this purpose. Polyvalent detection has allowed the detection of multiple plant viruses at the genus level. Multiplexing RT polymerase chain reaction (PCR) has been optimized for the simultaneous detection of more than 10 plant viruses/viroids. In this short review, we provide an update on the progress made during the last decade on techniques such as multiplex PCR, polyvalent PCR, non-isotopic molecular hybridization techniques, real-time PCR, and array technologies to allow simultaneous detection of multiple plant viruses. Also, the potential and benefits of the powerful new technique of deep sequencing/next-generation sequencing are described.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.