Evidence map›Paper›PMID 30250161›Full record

ArticleScientific reports2018

Development of a genome-informed loop-mediated isothermal amplification assay for rapid and specific detection of Xanthomonas euvesicatoria.

Adriana Larrea-Sarmiento, Upasana Dhakal, Gamze Boluk, Lilly Fatdal, Anne Alvarez, Amanda Strayer-Scherer, Mathews Paret, Jeff Jones, Daniel Jenkins, Mohammad Arif

Abstract read
In one paragraph

Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Adriana Larrea-SarmientoDepartment of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Honolulu, HI, United States.
Upasana DhakalDepartment of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Honolulu, HI, United States.
Gamze BolukDepartment of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Honolulu, HI, United States.
Lilly FatdalDepartment of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Honolulu, HI, United States.
Anne AlvarezDepartment of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Honolulu, HI, United States.
Amanda Strayer-SchererDepartment of Entomology and Plant Pathology, Mountain Research Station, North Carolina State University, Waynesville, NC, United States.
Mathews ParetDepartment of Plant Pathology, North Florida Research and Education Center, University of Florida, Quincy, FL, United States.
Jeff JonesDepartment of Plant Pathology, University of Florida, Gainesville, FL, United States.
Daniel JenkinsDepartment of Molecular Biosciences and BioEngineering, University of Hawaii at Manoa, Honolulu, HI, United States.
Mohammad ArifDepartment of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Honolulu, HI, United States. arif@hawaii.edu.ORCID 0000-0002-5887-2050

Funding

USDA | National Institute of Food and Agriculture (NIFA) 9038H
6 · The paper itself

Abstract

Bacterial spot (BS), caused by Xanthomonas euvesicatoria, X. vesicatoria, X. gardneri and X. perforans, is an economically important bacterial disease of tomato and pepper. Symptoms produced by all four species are nearly indistinguishable. At present, no point-of-care diagnostics exist for BS. In this research, we examined genomes of X. euvesicatoria, X. vesicatoria, X. gardneri, X. perforans and other species of Xanthomonas; the unique gene recG was chosen to design primers to develop a loop-mediated isothermal amplification (LAMP) assay to rapidly and accurately identify and differentiate X. euvesicatoria from other BS causing Xanthomonas sp. using a field-deployable portable BioRanger

Indexed as

Genome, BacterialComputer SimulationDNA PrimersGenetic VariationNucleic Acid Amplification TechniquesPhylogenyPlant DiseasesReproducibility of ResultsSolanum lycopersicumXanthomonasDNA Primers

Identifiers

PMID30250161
PMCPMC6155141

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.