ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2018
Duplex nested-PCR for detection of small ruminant lentiviruses.
Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 26 citations in OpenAlex.
- Small ruminant lentiviruses in dairy goats under tropical conditions: evidence of recombinant genotypes.Tropical animal health and production · 2026Article
- Molecular Characterization of Small Ruminant Lentiviruses in Sheep and Goats: A Systematic Review.Animals : an open access journal from MDPI · 2024Review
- Detection and Phylogenetic Analysis of Caprine Arthritis Encephalitis Virus Using TaqMan-based qPCR in Eastern China.Veterinary sciences · 2024Article
- In vivo evaluation of the antiretroviral activity of Melia azedarach against small ruminant lentiviruses in goat colostrum and milk.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Article
- The genetic variability of small-ruminant lentiviruses and its impact on tropism, the development of diagnostic tests and vaccines and the effectiveness of control programmes.Journal of veterinary research · 2023Article
- In vitro antiviral effect of ethanolic extracts from Azadirachta indica and Melia azedarach against goat lentivirus in colostrum and milk.Scientific reports · 2023Article
- Longitudinal Study on Seroreactivity of Goats Exposed to Colostrum and Milk of Small Ruminant Lentivirus-infected Dams.Journal of veterinary research · 2022Article
- First study on the molecular prevalence of caprine arthritis encephalitis virus in goats in Babylon, Iraq.Veterinary world · 2022Article
- Initial multi-target approach shows importance of improved caprine arthritis-encephalitis virus control program in Russia for hobbyist goat farms.Veterinary world · 2021Article
- Accurate Diagnosis of Small Ruminant Lentivirus Infection Is Needed for Selection of Resistant Sheep through TMEM154 E35K Genotyping.Pathogens (Basel, Switzerland) · 2021Article
- Vertical transmissibility of small ruminant lentivirus.PloS one · 2020Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Small ruminant lentiviruses (SRLV) have high genetic variability which results in different viral strains around the world. This create a challenge to design sensible primers for molecular diagnosis in different regions. This work proposes a protocol of duplex nested-PCR for the precise diagnosis of SRLV. The technique was designed and tested with the control strains CAEV Co and MVV 1514. Then, field strains were submitted to the same protocol of duplex nested-PCR. Blood samples of sheep and goats were tested with AGID and nested PCR with specific primers for pol, gag and LTR. The AGID results showed low detection capacity of positive animals, while the nested PCR demonstrated a greater capacity of virus detection. Results demonstrated that LTR-PCR was more efficient in detecting positive sheep samples, whereas gag-PCR allowed a good detection of samples of positive goats and positive sheep. In addition, pol-PCR was more efficient with goat samples than for sheep. Duplex nested PCR performed with standard virus samples and field strains demonstrated that the technique is more efficient for the detection of multiple pro-viral DNA sequences. This study demonstrated a successful duplex nested PCR assay allowing a more accurate diagnosis of SRLV.
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