ArticleBMC biology2018
Histone variants H2A.Z and H3.3 coordinately regulate PRC2-dependent H3K27me3 deposition and gene expression regulation in mES cells.
Article in BMC biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 83 citations in OpenAlex.
- Epigenetics in development, disease, and therapy: from mechanisms to clinical interventions.Science China. Life sciences · 2026Review
- Histone variant H2A.J is an epigenetic regulator of metastasis in lung adenocarcinoma.Cell death & disease · 2026Article
- H2A.Z is essential for oocyte maturation and fertility in female mouse.Nature structural & molecular biology · 2025Article
- H2A.Z reinforces maternal H3K4me3 formation and is essential for meiotic progression in mouse oocytes.Nature structural & molecular biology · 2025Article
- Ubinuclein 2 is essential for mouse development and functions in X chromosome inactivation.PLoS genetics · 2025Article
- The role of DNA content in shaping chromatin architecture and gene expression.The Plant journal : for cell and molecular biology · 2025Article
- Multifunctional histone variants in genome function.Nature reviews. Genetics · 2025Review
- Alternative silencing states of transposable elements in Arabidopsis associated with H3K27me3.Genome biology · 2025Article
- Epigenetic Regulatory Processes Involved in the Establishment and Maintenance of Skin Homeostasis-The Role of Microbiota.International journal of molecular sciences · 2025Review
- Characterizing the regulatory effects of H2A.Z and SWR1-C on gene expression during hydroxyurea exposure in Saccharomyces cerevisiae.PLoS genetics · 2025Article
- H2A.Z removal mediates the activation of genes accounting for cell elongation under light and temperature stress.Plant cell reports · 2024Review
- Plasma cell differentiation is regulated by the expression of histone variant H3.3.Nature communications · 2024Article
- Nucleation and spreading maintain Polycomb domains every cell cycle.Cell reports · 2024Article
- Anp32e protects against accumulation of H2A.Z at Sox motif containing promoters during zebrafish gastrulation.Developmental biology · 2024Article
- Long-Term Administration of Omeprazole-Induced Hypergastrinemia and Changed Glucose Homeostasis and Expression of Metabolism-Related Genes.BioMed research international · 2024Article
- Article
- Distinct roles for canonical and variant histone H3 lysine-36 in Polycomb silencing.Science advances · 2023Article
- Contribution of the histone variant H2A.Z to expression of responsive genes in plants.Seminars in cell & developmental biology · 2023Review
- Loss of MIR503HG facilitates papillary renal cell carcinoma associated lymphatic metastasis by triggering NOTCH1/VEGFC signaling.International journal of biological sciences · 2023Article
- Dynamical modeling of the H3K27 epigenetic landscape in mouse embryonic stem cells.PLoS computational biology · 2022Article
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Authors and funding
17 authors at 4 institutions in 2 countries.
Funding
Abstract
backgroundThe hierarchical organization of eukaryotic chromatin plays a central role in gene regulation, by controlling the extent to which the transcription machinery can access DNA. The histone variants H3.3 and H2A.Z have recently been identified as key regulatory players in this process, but the underlying molecular mechanisms by which they permit or restrict gene expression remain unclear. Here, we investigated the regulatory function of H3.3 and H2A.Z on chromatin dynamics and Polycomb-mediated gene silencing.
resultsOur ChIP-seq analysis reveals that in mouse embryonic stem (mES) cells, H3K27me3 enrichment correlates strongly with H2A.Z. We further demonstrate that H2A.Z promotes PRC2 activity on H3K27 methylation through facilitating chromatin compaction both in vitro and in mES cells. In contrast, PRC2 activity is counteracted by H3.3 through impairing chromatin compaction. However, a subset of H3.3 may positively regulate PRC2-dependent H3K27 methylation via coordinating depositions of H2A.Z to developmental and signaling genes in mES cells. Using all-trans retinoic acid (tRA)-induced gene as a model, we show that the dynamic deposition of H2A.Z and H3.3 coordinately regulates the PRC2-dependent H3K27 methylation by modulating local chromatin structure at the promoter region during the process of turning genes off.
conclusionsOur study provides key insights into the mechanism of how histone variants H3.3 and H2A.Z function coordinately to finely tune the PRC2 enzymatic activity during gene silencing, through promoting or impairing chromosome compaction respectively.
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