ArticleBMC genomics2018
Analysis of Drosophila melanogaster testis transcriptome.
Article in BMC genomics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed.
- Uncovering the long non-coding RNAs in Drosophila: Tissue specific expressions and functional roles in reproductive development.Molecular biology reports · 2026Review
- Pervasive relaxed selection on spermatogenesis genes coincident with the evolution of polygyny in gorillas.eLife · 2026Article
- Machine learning analysis of Drosophila testis transcriptomic data reveals potential regulatory sequences.BioData mining · 2026Article
- Metabolic Regulation in the Maintenance ofInternational journal of molecular sciences · 2026Review
- The C-terminal ZZ domain of the Drosophila ORB2 RNA-binding protein is required for spermatid individualization.G3 (Bethesda, Md.) · 2026Article
- Tom70-mediated mitochondria-nuclear envelope contacts regulate nuclear pore complex inheritance during gametogenesis.bioRxiv : the preprint server for biology · 2025Article
- Analysis of Transcript Expression and Core Promoter DNA Sequences of Brain, Adipose Tissues and Testis in Human and Fruit Fly.International journal of molecular sciences · 2025Article
- Oral intake of aripiprazole compromises male fertility in Drosophila.Biology direct · 2025Article
- Evolutionary Analysis of Gene-Expression Localization in the Model Crustacean, Daphnia pulex.Molecular ecology · 2025Article
- Cell type- and chromosome-specific chromatin landscapes and DNA replication programs ofbioRxiv : the preprint server for biology · 2025Article
- Single-cell consequences of X-linked meiotic drive in stalk-eyed flies.PLoS genetics · 2025Article
- Meiotic Sex Chromosome Inactivation: Conservation across the Drosophila genus.PLoS genetics · 2025Article
- Article
- A polycistronic locus encodes both a tumor-suppressive microRNA and a long non-coding RNA regulating testis morphology in Drosophila.bioRxiv : the preprint server for biology · 2025Article
- An orphan gene is essential for efficient sperm entry into eggs in Drosophila melanogaster.Genetics · 2025Article
- Article
- An orphan gene is essential for efficient sperm entry into eggs inbioRxiv : the preprint server for biology · 2024Article
- Versatile gamma-tubulin complexes contribute to the dynamic organization of MTOCs during Drosophila spermatogenesis.Communications biology · 2024Article
- The conserved genetic program of male germ cells uncovers ancient regulators of human spermatogenesis.eLife · 2024Article
- Novel function of U7 snRNA in the repression of HERV1/LTR12s and lincRNAs in human cells.Nucleic acids research · 2024Article
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Authors and funding
7 authors.
Funding
Abstract
backgroundThe formation of matured and individual sperm involves a series of molecular and spectacular morphological changes of the developing cysts in Drosophila melanogaster testis. Recent advances in RNA Sequencing (RNA-Seq) technology help us to understand the complexity of eukaryotic transcriptomes by dissecting different tissues and developmental stages of organisms. To gain a better understanding of cellular differentiation of spermatogenesis, we applied RNA-Seq to analyse the testis-specific transcriptome, including coding and non-coding genes.
resultsWe isolated three different parts of the wild-type testis by dissecting and cutting the different regions: 1.) the apical region, which contains stem cells and developing spermatocytes 2.) the middle region, with enrichment of meiotic cysts 3.) the basal region, which contains elongated post-meiotic cysts with spermatids. Total RNA was isolated from each region and analysed by next-generation sequencing. We collected data from the annotated 17412 Drosophila genes and identified 5381 genes with significant transcript accumulation differences between the regions, representing the main stages of spermatogenesis. We demonstrated for the first time the presence and region specific distribution of 2061 lncRNAs in testis, with 203 significant differences. Using the available modENCODE RNA-Seq data, we determined the tissue specificity indices of Drosophila genes. Combining the indices with our results, we identified genes with region-specific enrichment in testis.
conclusionBy multiple analyses of our results and integrating existing knowledge about Drosophila melanogaster spermatogenesis to our dataset, we were able to describe transcript composition of different regions of Drosophila testis, including several stage-specific transcripts. We present searchable visualizations that can facilitate the identification of new components that play role in the organisation and composition of different stages of spermatogenesis, including the less known, but complex regulation of post-meiotic stages.
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